Stabilization of supramolecular membrane protein–lipid bilayer assemblies through immobilization in a crystalline exoskeleton

Fabian C. Herbert, Sameera S. Abeyrathna, Nisansala S. Abeyrathna, Yalini H. Wijesundara, Olivia R. Brohlin, Francesco Carraro, Heinz Amenitsch, Paolo Falcaro, Michael A. Luzuriaga, Alejandra Durand-Silva, Shashini D. Diwakara, Ronald A. Smaldone, Gabriele Meloni () and Jeremiah J. Gassensmith ()
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Fabian C. Herbert: The University of Texas at Dallas
Sameera S. Abeyrathna: The University of Texas at Dallas
Nisansala S. Abeyrathna: The University of Texas at Dallas
Yalini H. Wijesundara: The University of Texas at Dallas
Olivia R. Brohlin: The University of Texas at Dallas
Francesco Carraro: Graz University of Technology
Heinz Amenitsch: Graz University of Technology
Paolo Falcaro: Graz University of Technology
Michael A. Luzuriaga: The University of Texas at Dallas
Alejandra Durand-Silva: The University of Texas at Dallas
Shashini D. Diwakara: The University of Texas at Dallas
Ronald A. Smaldone: The University of Texas at Dallas
Gabriele Meloni: The University of Texas at Dallas
Jeremiah J. Gassensmith: The University of Texas at Dallas

Nature Communications, 2021, vol. 12, issue 1, 1-13

Abstract: Abstract Artificial native-like lipid bilayer systems constructed from phospholipids assembling into unilamellar liposomes allow the reconstitution of detergent-solubilized transmembrane proteins into supramolecular lipid-protein assemblies called proteoliposomes, which mimic cellular membranes. Stabilization of these complexes remains challenging because of their chemical composition, the hydrophobicity and structural instability of membrane proteins, and the lability of interactions between protein, detergent, and lipids within micelles and lipid bilayers. In this work we demonstrate that metastable lipid, protein-detergent, and protein-lipid supramolecular complexes can be successfully generated and immobilized within zeolitic-imidazole framework (ZIF) to enhance their stability against chemical and physical stressors. Upon immobilization in ZIF bio-composites, blank liposomes, and model transmembrane metal transporters in detergent micelles or embedded in proteoliposomes resist elevated temperatures, exposure to chemical denaturants, aging, and mechanical stresses. Extensive morphological and functional characterization of the assemblies upon exfoliation reveal that all these complexes encapsulated within the framework maintain their native morphology, structure, and activity, which is otherwise lost rapidly without immobilization.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22285-y

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DOI: 10.1038/s41467-021-22285-y

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