Astigmatic traction force microscopy (aTFM)

Di Li, Huw Colin-York, Liliana Barbieri, Yousef Javanmardi, Yuting Guo, Kseniya Korobchevskaya, Emad Moeendarbary (), Dong Li () and Marco Fritzsche ()
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Di Li: Chinese Academy of Sciences
Huw Colin-York: University of Oxford
Liliana Barbieri: University of Oxford
Yousef Javanmardi: University College London
Yuting Guo: Chinese Academy of Sciences
Kseniya Korobchevskaya: University of Oxford
Emad Moeendarbary: University College London
Dong Li: Chinese Academy of Sciences
Marco Fritzsche: University of Oxford

Nature Communications, 2021, vol. 12, issue 1, 1-10

Abstract: Abstract Quantifying small, rapidly progressing three-dimensional forces generated by cells remains a major challenge towards a more complete understanding of mechanobiology. Traction force microscopy is one of the most broadly applied force probing technologies but ascertaining three-dimensional information typically necessitates slow, multi-frame z-stack acquisition with limited sensitivity. Here, by performing traction force microscopy using fast single-frame astigmatic imaging coupled with total internal reflection fluorescence microscopy we improve the temporal resolution of three-dimensional mechanical force quantification up to 10-fold compared to its related super-resolution modalities. 2.5D astigmatic traction force microscopy (aTFM) thus enables live-cell force measurements approaching physiological sensitivity.

Date: 2021
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DOI: 10.1038/s41467-021-22376-w

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