Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)

Liliana Barbieri, Huw Colin-York, Kseniya Korobchevskaya, Di Li, Deanna L. Wolfson, Narain Karedla, Falk Schneider, Balpreet S. Ahluwalia, Tore Seternes, Roy A. Dalmo, Michael L. Dustin, Dong Li () and Marco Fritzsche ()
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Liliana Barbieri: University of Oxford
Huw Colin-York: University of Oxford
Kseniya Korobchevskaya: University of Oxford
Di Li: Chinese Academy of Sciences
Deanna L. Wolfson: UiT The Arctic University of Norway
Narain Karedla: University of Oxford
Falk Schneider: University of Oxford
Balpreet S. Ahluwalia: UiT The Arctic University of Norway
Tore Seternes: UiT The Arctic University of Norway
Roy A. Dalmo: UiT The Arctic University of Norway
Michael L. Dustin: University of Oxford
Dong Li: Chinese Academy of Sciences
Marco Fritzsche: University of Oxford

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract Quantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.

Date: 2021
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DOI: 10.1038/s41467-021-22377-9

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