Regulation of Arabidopsis photoreceptor CRY2 by two distinct E3 ubiquitin ligases

Yadi Chen, Xiaohua Hu, Siyuan Liu, Tiantian Su, Hsiaochi Huang, Huibo Ren, Zhensheng Gao, Xu Wang, Deshu Lin, James A. Wohlschlegel, Qin Wang () and Chentao Lin
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Yadi Chen: Fujian Agriculture and Forestry University
Xiaohua Hu: Fujian Agriculture and Forestry University
Siyuan Liu: Fujian Agriculture and Forestry University
Tiantian Su: University of California
Hsiaochi Huang: University of California
Huibo Ren: Fujian Agriculture and Forestry University
Zhensheng Gao: Fujian Agriculture and Forestry University
Xu Wang: Fujian Agriculture and Forestry University
Deshu Lin: Fujian Agriculture and Forestry University
James A. Wohlschlegel: University of California
Qin Wang: Fujian Agriculture and Forestry University
Chentao Lin: University of California

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract Cryptochromes (CRYs) are photoreceptors or components of the molecular clock in various evolutionary lineages, and they are commonly regulated by polyubiquitination and proteolysis. Multiple E3 ubiquitin ligases regulate CRYs in animal models, and previous genetics study also suggest existence of multiple E3 ubiquitin ligases for plant CRYs. However, only one E3 ligase, Cul4COP1/SPAs, has been reported for plant CRYs so far. Here we show that Cul3LRBs is the second E3 ligase of CRY2 in Arabidopsis. We demonstrate the blue light-specific and CRY-dependent activity of LRBs (Light-Response Bric-a-Brack/Tramtrack/Broad 1, 2 & 3) in blue-light regulation of hypocotyl elongation. LRBs physically interact with photoexcited and phosphorylated CRY2, at the CCE domain of CRY2, to facilitate polyubiquitination and degradation of CRY2 in response to blue light. We propose that Cul4COP1/SPAs and Cul3LRBs E3 ligases interact with CRY2 via different structure elements to regulate the abundance of CRY2 photoreceptor under different light conditions, facilitating optimal photoresponses of plants grown in nature.

Date: 2021
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DOI: 10.1038/s41467-021-22410-x

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