Direct 1,3-butadiene biosynthesis in Escherichia coli via a tailored ferulic acid decarboxylase mutant

Yutaro Mori, Shuhei Noda, Tomokazu Shirai () and Akihiko Kondo
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Yutaro Mori: Center for Sustainable Resource Science, RIKEN
Shuhei Noda: Center for Sustainable Resource Science, RIKEN
Tomokazu Shirai: Center for Sustainable Resource Science, RIKEN
Akihiko Kondo: Center for Sustainable Resource Science, RIKEN

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract The C4 unsaturated compound 1,3-butadiene is an important monomer in synthetic rubber and engineering plastic production. However, microorganisms cannot directly produce 1,3-butadiene when glucose is used as a renewable carbon source via biological processes. In this study, we construct an artificial metabolic pathway for 1,3-butadiene production from glucose in Escherichia coli by combining the cis,cis-muconic acid (ccMA)-producing pathway together with tailored ferulic acid decarboxylase mutations. The rational design of the substrate-binding site of the enzyme by computational simulations improves ccMA decarboxylation and thus 1,3-butadiene production. We find that changing dissolved oxygen (DO) levels and controlling the pH are important factors for 1,3-butadiene production. Using DO–stat fed-batch fermentation, we produce 2.13 ± 0.17 g L−1 1,3-butadiene. The results indicate that we can produce unnatural/nonbiological compounds from glucose as a renewable carbon source via a rational enzyme design strategy.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22504-6

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DOI: 10.1038/s41467-021-22504-6

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