Super-resolved visualization of single DNA-based tension sensors in cell adhesion

Thomas Schlichthaerle, Caroline Lindner and Ralf Jungmann ()
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Thomas Schlichthaerle: Ludwig Maximilian University
Caroline Lindner: Ludwig Maximilian University
Ralf Jungmann: Ludwig Maximilian University

Nature Communications, 2021, vol. 12, issue 1, 1-8

Abstract: Abstract Cell-extracellular matrix sensing plays a crucial role in cellular behavior and leads to the formation of a macromolecular protein complex called the focal adhesion. Despite their importance in cellular decision making, relatively little is known about cell-matrix interactions and the intracellular transduction of an initial ligand-receptor binding event on the single-molecule level. Here, we combine cRGD-ligand-decorated DNA tension sensors with DNA-PAINT super-resolution microscopy to study the mechanical engagement of single integrin receptors and the downstream influence on actin bundling. We uncover that integrin receptor clustering is governed by a non-random organization with complexes spaced at 20–30 nm distances. The DNA-based tension sensor and analysis framework provide powerful tools to study a multitude of receptor-ligand interactions where forces are involved in ligand-receptor binding.

Date: 2021
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DOI: 10.1038/s41467-021-22606-1

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