Inhibition of polar actin assembly by astral microtubules is required for cytokinesis

Anan Chen, Luisa Ulloa Severino, Thomas C. Panagiotou, Trevor F. Moraes, Darren A. Yuen, Brigitte D. Lavoie and Andrew Wilde ()
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Anan Chen: University of Toronto
Luisa Ulloa Severino: Keenan Research Centre for Biomedical Science, Li Ka Shing Knowledge Institute
Thomas C. Panagiotou: University of Toronto
Trevor F. Moraes: University of Toronto
Darren A. Yuen: Keenan Research Centre for Biomedical Science, Li Ka Shing Knowledge Institute
Brigitte D. Lavoie: University of Toronto
Andrew Wilde: University of Toronto

Nature Communications, 2021, vol. 12, issue 1, 1-13

Abstract: Abstract During cytokinesis, the actin cytoskeleton is partitioned into two spatially distinct actin isoform specific networks: a β-actin network that generates the equatorial contractile ring, and a γ-actin network that localizes to the cell cortex. Here we demonstrate that the opposing regulation of the β- and γ-actin networks is required for successful cytokinesis. While activation of the formin DIAPH3 at the cytokinetic furrow underlies β-actin filament production, we show that the γ-actin network is specifically depleted at the cell poles through the localized deactivation of the formin DIAPH1. During anaphase, CLIP170 is delivered by astral microtubules and displaces IQGAP1 from DIAPH1, leading to formin autoinhibition, a decrease in cortical stiffness and localized membrane blebbing. The contemporaneous production of a β-actin contractile ring at the cell equator and loss of γ-actin from the poles is required to generate a stable cytokinetic furrow and for the completion of cell division.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22677-0

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DOI: 10.1038/s41467-021-22677-0

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