Structural studies of phosphorylation-dependent interactions between the V2R receptor and arrestin-2

Qing-Tao He, Peng Xiao, Shen-Ming Huang, Ying-Li Jia, Zhong-Liang Zhu, Jing-Yu Lin, Fan Yang, Xiao-Na Tao, Ru-Jia Zhao, Feng-Yuan Gao, Xiao-Gang Niu, Kun-Hong Xiao, Jiangyun Wang (), Changwen Jin (), Jin-Peng Sun () and Xiao Yu ()
Additional contact information
Qing-Tao He: Shandong University
Peng Xiao: Shandong University
Shen-Ming Huang: Peking University
Ying-Li Jia: Peking University
Zhong-Liang Zhu: University of Science and Technology of China
Jing-Yu Lin: Peking University
Fan Yang: Shandong University
Xiao-Na Tao: Shandong University
Ru-Jia Zhao: Shandong University
Feng-Yuan Gao: Shandong University
Xiao-Gang Niu: Peking University
Kun-Hong Xiao: University of Pittsburgh
Jiangyun Wang: Chinese Academy of Sciences
Changwen Jin: Peking University
Jin-Peng Sun: Shandong University
Xiao Yu: Shandong University

Nature Communications, 2021, vol. 12, issue 1, 1-16

Abstract: Abstract Arrestins recognize different receptor phosphorylation patterns and convert this information to selective arrestin functions to expand the functional diversity of the G protein-coupled receptor (GPCR) superfamilies. However, the principles governing arrestin-phospho-receptor interactions, as well as the contribution of each single phospho-interaction to selective arrestin structural and functional states, are undefined. Here, we determined the crystal structures of arrestin2 in complex with four different phosphopeptides derived from the vasopressin receptor-2 (V2R) C-tail. A comparison of these four crystal structures with previously solved Arrestin2 structures demonstrated that a single phospho-interaction change results in measurable conformational changes at remote sites in the complex. This conformational bias introduced by specific phosphorylation patterns was further inspected by FRET and 1H NMR spectrum analysis facilitated via genetic code expansion. Moreover, an interdependent phospho-binding mechanism of phospho-receptor-arrestin interactions between different phospho-interaction sites was unexpectedly revealed. Taken together, our results provide evidence showing that phospho-interaction changes at different arrestin sites can elicit changes in affinity and structural states at remote sites, which correlate with selective arrestin functions.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22731-x

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DOI: 10.1038/s41467-021-22731-x

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