Portable bioluminescent platform for in vivo monitoring of biological processes in non-transgenic animals

Aleksey Yevtodiyenko, Arkadiy Bazhin, Pavlo Khodakivskyi, Aurelien Godinat, Ghyslain Budin, Tamara Maric, Giorgio Pietramaggiori, Sandra S. Scherer, Marina Kunchulia, George Eppeldauer, Sergey V. Polyakov, Kevin P. Francis, Jeffrey N. Bryan and Elena A. Goun ()
Additional contact information
Aleksey Yevtodiyenko: Swiss Federal Institute of Technology (EPFL)
Arkadiy Bazhin: Swiss Federal Institute of Technology (EPFL)
Pavlo Khodakivskyi: Swiss Federal Institute of Technology (EPFL)
Aurelien Godinat: Swiss Federal Institute of Technology (EPFL)
Ghyslain Budin: Swiss Federal Institute of Technology (EPFL)
Tamara Maric: Swiss Federal Institute of Technology (EPFL)
Giorgio Pietramaggiori: Plastic and Reconstructive Surgery, Global Plastic Surgery
Sandra S. Scherer: Plastic and Reconstructive Surgery, Global Plastic Surgery
Marina Kunchulia: Free University of Tbilisi
George Eppeldauer: National Institute of Standards and Technology (NIST)
Sergey V. Polyakov: National Institute of Standards and Technology (NIST)
Kevin P. Francis: David Geffen School of Medicine at UCLA
Jeffrey N. Bryan: University of Missouri-Columbia
Elena A. Goun: Swiss Federal Institute of Technology (EPFL)

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract Bioluminescent imaging (BLI) is one of the most powerful and widely used preclinical imaging modalities. However, the current technology relies on the use of transgenic luciferase-expressing cells and animals and therefore can only be applied to a limited number of existing animal models of human disease. Here, we report the development of a “portable bioluminescent” (PBL) technology that overcomes most of the major limitations of traditional BLI. We demonstrate that the PBL method is capable of noninvasive measuring the activity of both extracellular (e.g., dipeptidyl peptidase 4) and intracellular (e.g., cytochrome P450) enzymes in vivo in non-luciferase-expressing mice. Moreover, we successfully utilize PBL technology in dogs and human cadaver, paving the way for the translation of functional BLI to the noninvasive quantification of biological processes in large animals. The PBL methodology can be easily adapted for the noninvasive monitoring of a plethora of diseases across multiple species.

Date: 2021
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/s41467-021-22892-9 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-22892-9

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-021-22892-9

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().