The RNA binding protein FgRbp1 regulates specific pre-mRNA splicing via interacting with U2AF23 in Fusarium

Minhui Wang, Tianling Ma, Haixia Wang, Jianzhao Liu, Yun Chen (), Won Bo Shim () and Zhonghua Ma ()
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Minhui Wang: Institute of Biotechnology, Zhejiang University
Tianling Ma: Institute of Biotechnology, Zhejiang University
Haixia Wang: Institute of Biotechnology, Zhejiang University
Jianzhao Liu: Zhejiang University
Yun Chen: Institute of Biotechnology, Zhejiang University
Won Bo Shim: Texas A&M University
Zhonghua Ma: Institute of Biotechnology, Zhejiang University

Nature Communications, 2021, vol. 12, issue 1, 1-15

Abstract: Abstract Precursor messenger RNA (pre-mRNA) splicing is an essential and tightly regulated process in eukaryotic cells; however, the regulatory mechanisms for the splicing are not well understood. Here, we characterize a RNA binding protein named FgRbp1 in Fusarium graminearum, a fungal pathogen of cereal crops worldwide. Deletion of FgRbp1 leads to reduced splicing efficiency in 47% of the F. graminearum intron-containing gene transcripts that are involved in various cellular processes including vegetative growth, development, and virulence. The human ortholog RBM42 is able to fully rescue the growth defects of ΔFgRbp1. FgRbp1 binds to the motif CAAGR in its target mRNAs, and interacts with the splicing factor FgU2AF23, a highly conserved protein involved in 3’ splice site recognition, leading to enhanced recruitment of FgU2AF23 to the target mRNAs. This study demonstrates that FgRbp1 is a splicing regulator and regulates the pre-mRNA splicing in a sequence-dependent manner in F. graminearum.

Date: 2021
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DOI: 10.1038/s41467-021-22917-3

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