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A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

Guanhua Xun, Stephan Thomas Lane, Vassily Andrew Petrov, Brandon Elliott Pepa and Huimin Zhao ()
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Guanhua Xun: University of Illinois at Urbana-Champaign
Stephan Thomas Lane: Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign
Vassily Andrew Petrov: Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign
Brandon Elliott Pepa: University of Illinois at Urbana-Champaign
Huimin Zhao: University of Illinois at Urbana-Champaign

Nature Communications, 2021, vol. 12, issue 1, 1-9

Abstract: Abstract The need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities.

Date: 2021
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DOI: 10.1038/s41467-021-23185-x

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