RETRACTED ARTICLE: Production of viable chicken by allogeneic transplantation of primordial germ cells induced from somatic cells

Ruifeng Zhao, Qisheng Zuo, Xia Yuan, Kai Jin, Jing Jin, Ying Ding, Chen Zhang, Tingting Li, Jingyi Jiang, Jiancheng Li, Ming Zhang, Xiang Shi, Hongyan Sun, Yani Zhang, Qi Xu, Guobin Chang, Zhenhua Zhao, Bing Li, Xinsheng Wu, Yang Zhang, Jiuzhou Song, Guohong Chen () and Bichun Li ()
Additional contact information
Ruifeng Zhao: Yangzhou University
Qisheng Zuo: Yangzhou University
Xia Yuan: Yangzhou University
Kai Jin: Yangzhou University
Jing Jin: Yangzhou University
Ying Ding: Yangzhou University
Chen Zhang: Yangzhou University
Tingting Li: Yangzhou University
Jingyi Jiang: Yangzhou University
Jiancheng Li: Yangzhou University
Ming Zhang: Yangzhou University
Xiang Shi: Yangzhou University
Hongyan Sun: Yangzhou University
Yani Zhang: Yangzhou University
Qi Xu: Yangzhou University
Guobin Chang: Yangzhou University
Zhenhua Zhao: The Poultry Research Institute of Chinese Academy of Agricultural Sciences
Bing Li: The Poultry Research Institute of Chinese Academy of Agricultural Sciences
Xinsheng Wu: Yangzhou University
Yang Zhang: Yangzhou University
Jiuzhou Song: University of Maryland
Guohong Chen: Yangzhou University
Bichun Li: Yangzhou University

Nature Communications, 2021, vol. 12, issue 1, 1-13

Abstract: Abstract The allogeneic transplantation of primordial germ cells (PGCs) derived from somatic cells overcomes the limitation of avian cloning. Here, we transdifferentiate chicken embryo fibroblasts (CEFs) from black feathered Langshan chickens to PGCs and transplant them into White Plymouth Rock chicken embryos to produce viable offspring with characteristics inherited from the donor. We express Oct4/Sox2/Nanog/Lin28A (OSNL) to reprogram CEFs to induced pluripotent stem cells (iPSCs), which are further induced to differentiate into PGCs by BMP4/BMP8b/EGF. DNA demethylation, histone acetylation and glycolytic activation elevate the iPSC induction efficiency, while histone acetylation and glycolytic inhibition facilitate PGCs formation. The induced PGCs (iPGCs) are transplanted into the recipients, which are self-crossed to produce 189/509 somatic cells derived chicken with the donor’s characteristics. Microsatellite analysis and genome sequencing confirm the inheritance of genetic information from the donor. Thus, we demonstrate the feasibility of avian cloning from somatic cells.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23242-5

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DOI: 10.1038/s41467-021-23242-5

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