Genome-resolved metagenomics reveals role of iron metabolism in drought-induced rhizosphere microbiome dynamics

Ling Xu (), Zhaobin Dong, Dawn Chiniquy, Grady Pierroz, Siwen Deng, Cheng Gao, Spencer Diamond, Tuesday Simmons, Heidi M.-L. Wipf, Daniel Caddell, Nelle Varoquaux, Mary A. Madera, Robert Hutmacher, Adam Deutschbauer, Jeffery A. Dahlberg, Mary Lou Guerinot, Elizabeth Purdom, Jillian F. Banfield, John W. Taylor, Peggy G. Lemaux and Devin Coleman-Derr ()
Additional contact information
Ling Xu: University of California
Zhaobin Dong: University of California
Dawn Chiniquy: Lawrence Berkeley National Laboratory
Grady Pierroz: University of California
Siwen Deng: University of California
Cheng Gao: University of California
Spencer Diamond: University of California
Tuesday Simmons: University of California
Heidi M.-L. Wipf: University of California
Daniel Caddell: Plant Gene Expression Center, USDA-ARS
Nelle Varoquaux: CNRS, University Grenoble Alpes, TIMC-IMAG
Mary A. Madera: University of California
Robert Hutmacher: University of California
Adam Deutschbauer: Lawrence Berkeley National Laboratory
Jeffery A. Dahlberg: Kearney Agricultural Research & Extension Center
Mary Lou Guerinot: Dartmouth College
Elizabeth Purdom: University of California
Jillian F. Banfield: University of California
John W. Taylor: University of California
Peggy G. Lemaux: University of California
Devin Coleman-Derr: University of California

Nature Communications, 2021, vol. 12, issue 1, 1-17

Abstract: Abstract Recent studies have demonstrated that drought leads to dramatic, highly conserved shifts in the root microbiome. At present, the molecular mechanisms underlying these responses remain largely uncharacterized. Here we employ genome-resolved metagenomics and comparative genomics to demonstrate that carbohydrate and secondary metabolite transport functionalities are overrepresented within drought-enriched taxa. These data also reveal that bacterial iron transport and metabolism functionality is highly correlated with drought enrichment. Using time-series root RNA-Seq data, we demonstrate that iron homeostasis within the root is impacted by drought stress, and that loss of a plant phytosiderophore iron transporter impacts microbial community composition, leading to significant increases in the drought-enriched lineage, Actinobacteria. Finally, we show that exogenous application of iron disrupts the drought-induced enrichment of Actinobacteria, as well as their improvement in host phenotype during drought stress. Collectively, our findings implicate iron metabolism in the root microbiome’s response to drought and may inform efforts to improve plant drought tolerance to increase food security.

Date: 2021
References: Add references at CitEc
Citations: View citations in EconPapers (4)

Downloads: (external link)
https://www.nature.com/articles/s41467-021-23553-7 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23553-7

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-021-23553-7

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().