A recombined Sr26 and Sr61 disease resistance gene stack in wheat encodes unrelated NLR genes

Jianping Zhang, Timothy C. Hewitt, Willem H. P. Boshoff, Ian Dundas, Narayana Upadhyaya, Jianbo Li, Mehran Patpour, Sutha Chandramohan, Zacharias A. Pretorius, Mogens Hovmøller, Wendelin Schnippenkoetter, Robert F. Park, Rohit Mago, Sambasivam Periyannan, Dhara Bhatt, Sami Hoxha, Soma Chakraborty, Ming Luo, Peter Dodds, Burkhard Steuernagel, Brande B. H. Wulff, Michael Ayliffe, Robert A. McIntosh, Peng Zhang () and Evans S. Lagudah ()
Additional contact information
Jianping Zhang: University of Sydney
Timothy C. Hewitt: University of Sydney
Willem H. P. Boshoff: University of the Free State
Ian Dundas: University of Adelaide
Narayana Upadhyaya: CSIRO Agriculture & Food
Jianbo Li: University of Sydney
Mehran Patpour: Aarhus University
Sutha Chandramohan: CSIRO Agriculture & Food
Zacharias A. Pretorius: University of the Free State
Mogens Hovmøller: Aarhus University
Wendelin Schnippenkoetter: CSIRO Agriculture & Food
Robert F. Park: University of Sydney
Rohit Mago: CSIRO Agriculture & Food
Sambasivam Periyannan: CSIRO Agriculture & Food
Dhara Bhatt: CSIRO Agriculture & Food
Sami Hoxha: University of Sydney
Soma Chakraborty: CSIRO Agriculture & Food
Ming Luo: CSIRO Agriculture & Food
Peter Dodds: CSIRO Agriculture & Food
Burkhard Steuernagel: John Innes Centre, Norwich Research Park
Brande B. H. Wulff: John Innes Centre, Norwich Research Park
Michael Ayliffe: CSIRO Agriculture & Food
Robert A. McIntosh: University of Sydney
Peng Zhang: University of Sydney
Evans S. Lagudah: University of Sydney

Nature Communications, 2021, vol. 12, issue 1, 1-8

Abstract: Abstract The re-emergence of stem rust on wheat in Europe and Africa is reinforcing the ongoing need for durable resistance gene deployment. Here, we isolate from wheat, Sr26 and Sr61, with both genes independently introduced as alien chromosome introgressions from tall wheat grass (Thinopyrum ponticum). Mutational genomics and targeted exome capture identify Sr26 and Sr61 as separate single genes that encode unrelated (34.8%) nucleotide binding site leucine rich repeat proteins. Sr26 and Sr61 are each validated by transgenic complementation using endogenous and/or heterologous promoter sequences. Sr61 orthologs are absent from current Thinopyrum elongatum and wheat pan genome sequences, contrasting with Sr26 where homologues are present. Using gene-specific markers, we validate the presence of both genes on a single recombinant alien segment developed in wheat. The co-location of these genes on a small non-recombinogenic segment simplifies their deployment as a gene stack and potentially enhances their resistance durability.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-23738-0

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DOI: 10.1038/s41467-021-23738-0

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