Intracellular artificial supramolecules based on de novo designed Y15 peptides

Takayuki Miki (), Taichi Nakai, Masahiro Hashimoto, Keigo Kajiwara, Hiroshi Tsutsumi and Hisakazu Mihara
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Takayuki Miki: Tokyo Institute of Technology
Taichi Nakai: Tokyo Institute of Technology
Masahiro Hashimoto: Tokyo Institute of Technology
Keigo Kajiwara: Tokyo Institute of Technology
Hiroshi Tsutsumi: Tokyo Institute of Technology
Hisakazu Mihara: Tokyo Institute of Technology

Nature Communications, 2021, vol. 12, issue 1, 1-11

Abstract: Abstract De novo designed self-assembling peptides (SAPs) are promising building blocks of supramolecular biomaterials, which can fulfill a wide range of applications, such as scaffolds for tissue culture, three-dimensional cell culture, and vaccine adjuvants. Nevertheless, the use of SAPs in intracellular spaces has mostly been unexplored. Here, we report a self-assembling peptide, Y15 (YEYKYEYKYEYKYEY), which readily forms β-sheet structures to facilitate bottom-up synthesis of functional protein assemblies in living cells. Superfolder green fluorescent protein (sfGFP) fused to Y15 assembles into fibrils and is observed as fluorescent puncta in mammalian cells. Y15 self-assembly is validated by fluorescence anisotropy and pull-down assays. By using the Y15 platform, we demonstrate intracellular reconstitution of Nck assembly, a Src-homology 2 and 3 domain-containing adaptor protein. The artificial clusters of Nck induce N-WASP (neural Wiskott-Aldrich syndrome protein)-mediated actin polymerization, and the functional importance of Nck domain valency and density is evaluated.

Date: 2021
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DOI: 10.1038/s41467-021-23794-6

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