High-throughput screening and rational design of biofunctionalized surfaces with optimized biocompatibility and antimicrobial activity

Zhou Fang, Junjian Chen, Ye Zhu, Guansong Hu, Haoqian Xin, Kunzhong Guo, Qingtao Li, Liangxu Xie, Lin Wang (), Xuetao Shi (), Yingjun Wang () and Chuanbin Mao ()
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Zhou Fang: South China University of Technology
Junjian Chen: South China University of Technology
Ye Zhu: University of Oklahoma
Guansong Hu: South China University of Technology
Haoqian Xin: South China University of Technology
Kunzhong Guo: South China University of Technology
Qingtao Li: South China University of Technology
Liangxu Xie: Jiangsu University of Technology
Lin Wang: South China University of Technology
Xuetao Shi: South China University of Technology
Yingjun Wang: South China University of Technology
Chuanbin Mao: University of Oklahoma

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract Peptides are widely used for surface modification to develop improved implants, such as cell adhesion RGD peptide and antimicrobial peptide (AMP). However, it is a daunting challenge to identify an optimized condition with the two peptides showing their intended activities and the parameters for reaching such a condition. Herein, we develop a high-throughput strategy, preparing titanium (Ti) surfaces with a gradient in peptide density by click reaction as a platform, to screen the positions with desired functions. Such positions are corresponding to optimized molecular parameters (peptide densities/ratios) and associated preparation parameters (reaction times/reactant concentrations). These parameters are then extracted to prepare nongradient mono- and dual-peptide functionalized Ti surfaces with desired biocompatibility or/and antimicrobial activity in vitro and in vivo. We also demonstrate this strategy could be extended to other materials. Here, we show that the high-throughput versatile strategy holds great promise for rational design and preparation of functional biomaterial surfaces.

Date: 2021
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DOI: 10.1038/s41467-021-23954-8

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