Renewable fatty acid ester production in Clostridium

Jun Feng, Jie Zhang, Yuechao Ma, Yiming Feng, Shangjun Wang, Na Guo, Haijiao Wang, Pixiang Wang, Pablo Jiménez-Bonilla, Yanyan Gu, Junping Zhou, Zhong-Tian Zhang, Mingfeng Cao, Di Jiang, Shuning Wang, Xian-Wei Liu, Zengyi Shao, Ilya Borovok, Haibo Huang () and Yi Wang ()
Additional contact information
Jun Feng: Auburn University
Jie Zhang: Auburn University
Yuechao Ma: Auburn University
Yiming Feng: Virginia Tech
Shangjun Wang: Auburn University
Na Guo: Auburn University
Haijiao Wang: Auburn University
Pixiang Wang: Auburn University
Pablo Jiménez-Bonilla: Auburn University
Yanyan Gu: Auburn University
Junping Zhou: Auburn University
Zhong-Tian Zhang: Auburn University
Mingfeng Cao: Iowa State University
Di Jiang: University of Science and Technology of China
Shuning Wang: Microbial Technology Institute, Shandong University
Xian-Wei Liu: University of Science and Technology of China
Zengyi Shao: Iowa State University
Ilya Borovok: Tel Aviv University, Ramat Aviv
Haibo Huang: Virginia Tech
Yi Wang: Auburn University

Nature Communications, 2021, vol. 12, issue 1, 1-13

Abstract: Abstract Bioproduction of renewable chemicals is considered as an urgent solution for fossil energy crisis. However, despite tremendous efforts, it is still challenging to generate microbial strains that can produce target biochemical to high levels. Here, we report an example of biosynthesis of high-value and easy-recoverable derivatives built upon natural microbial pathways, leading to improvement in bioproduction efficiency. By leveraging pathways in solventogenic clostridia for co-producing acyl-CoAs, acids and alcohols as precursors, through rational screening for host strains and enzymes, systematic metabolic engineering-including elimination of putative prophages, we develop strains that can produce 20.3 g/L butyl acetate and 1.6 g/L butyl butyrate. Techno-economic analysis results suggest the economic competitiveness of our developed bioprocess. Our principles of selecting the most appropriate host for specific bioproduction and engineering microbial chassis to produce high-value and easy-separable end products may be applicable to other bioprocesses.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24038-3

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DOI: 10.1038/s41467-021-24038-3

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