Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato

Ernst-Jan Eggers, Ate Burgt, Sjaak A. W. Heusden, Michiel E. Vries, Richard G. F. Visser, Christian W. B. Bachem () and Pim Lindhout
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Ernst-Jan Eggers: Solynta, Dreijenlaan
Ate Burgt: Solynta, Dreijenlaan
Sjaak A. W. Heusden: Wageningen University & Research
Michiel E. Vries: Solynta, Dreijenlaan
Richard G. F. Visser: Wageningen University & Research
Christian W. B. Bachem: Wageningen University & Research
Pim Lindhout: Solynta, Dreijenlaan

Nature Communications, 2021, vol. 12, issue 1, 1-9

Abstract: Abstract Genetic gain in potato is hampered by the heterozygous tetraploid genome of cultivated potato. Converting potato into a diploid inbred-line based F1-hybrid crop provides a promising route towards increased genetic gain. The introduction of a dominant S-locus inhibitor (Sli) gene into diploid potato germplasm allows efficient generation of self-fertilized seeds and thus the development of potato inbred lines. Little is known about the structure and function of the Sli locus. Here we describe the mapping of Sli to a 12.6 kb interval on chromosome 12 using a recombinant screen approach. One of two candidate genes present in this interval shows a unique sequence that is exclusively present in self-compatible lines. We describe an expression vector that converts self-incompatible genotypes into self-compatible and a CRISPR-Cas9 vector that converts SC genotypes into SI. The Sli gene encodes an F-box protein that is specifically expressed in pollen from self-compatible plants. A 533 bp insertion in the promotor of that gene leads to a gain of function mutation, which overcomes self-pollen rejection.

Date: 2021
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DOI: 10.1038/s41467-021-24267-6

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