Fluid flow-induced left-right asymmetric decay of Dand5 mRNA in the mouse embryo requires a Bicc1-Ccr4 RNA degradation complex

Katsura Minegishi, Benjamin Rothé, Kaoru R. Komatsu, Hiroki Ono, Yayoi Ikawa, Hiromi Nishimura, Takanobu A. Katoh, Eriko Kajikawa, Xiaorei Sai, Emi Miyashita, Katsuyoshi Takaoka, Kana Bando, Hiroshi Kiyonari, Tadashi Yamamoto, Hirohide Saito (), Daniel B. Constam () and Hiroshi Hamada ()
Additional contact information
Katsura Minegishi: RIKEN Center for Biosystems Dynamics Research
Benjamin Rothé: School of Life Sciences
Kaoru R. Komatsu: Kyoto University
Hiroki Ono: Kyoto University
Yayoi Ikawa: RIKEN Center for Biosystems Dynamics Research
Hiromi Nishimura: RIKEN Center for Biosystems Dynamics Research
Takanobu A. Katoh: RIKEN Center for Biosystems Dynamics Research
Eriko Kajikawa: RIKEN Center for Biosystems Dynamics Research
Xiaorei Sai: RIKEN Center for Biosystems Dynamics Research
Emi Miyashita: Kyoto University
Katsuyoshi Takaoka: RIKEN Center for Biosystems Dynamics Research
Kana Bando: RIKEN Center for Biosystems Dynamics Research
Hiroshi Kiyonari: RIKEN Center for Biosystems Dynamics Research
Tadashi Yamamoto: Center for Integrative Medical Sciences
Hirohide Saito: Kyoto University
Daniel B. Constam: School of Life Sciences
Hiroshi Hamada: RIKEN Center for Biosystems Dynamics Research

Nature Communications, 2021, vol. 12, issue 1, 1-17

Abstract: Abstract Molecular left-right (L-R) asymmetry is established at the node of the mouse embryo as a result of the sensing of a leftward fluid flow by immotile cilia of perinodal crown cells and the consequent degradation of Dand5 mRNA on the left side. We here examined how the fluid flow induces Dand5 mRNA decay. We found that the first 200 nucleotides in the 3′ untranslated region (3′-UTR) of Dand5 mRNA are necessary and sufficient for the left-sided decay and to mediate the response of a 3′-UTR reporter transgene to Ca2+, the cation channel Pkd2, the RNA-binding protein Bicc1 and their regulation by the flow direction. We show that Bicc1 preferentially recognizes GACR and YGAC sequences, which can explain the specific binding to a conserved GACGUGAC motif located in the proximal Dand5 3′-UTR. The Cnot3 component of the Ccr4-Not deadenylase complex interacts with Bicc1 and is also required for Dand5 mRNA decay at the node. These results suggest that Ca2+ currents induced by leftward fluid flow stimulate Bicc1 and Ccr4-Not to mediate Dand5 mRNA degradation specifically on the left side of the node.

Date: 2021
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-021-24295-2 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24295-2

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-021-24295-2

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().