Genetic dissection identifies Necdin as a driver gene in a mouse model of paternal 15q duplications

Kota Tamada, Keita Fukumoto, Tsuyoshi Toya, Nobuhiro Nakai, Janak R. Awasthi, Shinji Tanaka, Shigeo Okabe, François Spitz, Fumihito Saitow, Hidenori Suzuki and Toru Takumi ()
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Kota Tamada: RIKEN Brain Science Institute, Wako
Keita Fukumoto: RIKEN Brain Science Institute, Wako
Tsuyoshi Toya: RIKEN Brain Science Institute, Wako
Nobuhiro Nakai: RIKEN Brain Science Institute, Wako
Janak R. Awasthi: RIKEN Brain Science Institute, Wako
Shinji Tanaka: The University of Tokyo, Bunkyo
Shigeo Okabe: The University of Tokyo, Bunkyo
François Spitz: University of Chicago
Fumihito Saitow: Garduate School of Medicine, Nippon Medical School, Bunkyo
Hidenori Suzuki: Garduate School of Medicine, Nippon Medical School, Bunkyo
Toru Takumi: RIKEN Brain Science Institute, Wako

Nature Communications, 2021, vol. 12, issue 1, 1-16

Abstract: Abstract Maternally inherited duplication of chromosome 15q11-q13 (Dup15q) is a pathogenic copy number variation (CNV) associated with autism spectrum disorder (ASD). Recently, paternally derived duplication has also been shown to contribute to the development of ASD. The molecular mechanism underlying paternal Dup15q remains unclear. Here, we conduct genetic and overexpression-based screening and identify Necdin (Ndn) as a driver gene for paternal Dup15q resulting in the development of ASD-like phenotypes in mice. An excess amount of Ndn results in enhanced spine formation and density as well as hyperexcitability of cortical pyramidal neurons. We generate 15q dupΔNdn mice with a normalized copy number of Ndn by excising its one copy from Dup15q mice using a CRISPR-Cas9 system. 15q dupΔNdn mice do not show ASD-like phenotypes and show dendritic spine dynamics and cortical excitatory-inhibitory balance similar to wild type animals. Our study provides an insight into the role of Ndn in paternal 15q duplication and a mouse model of paternal Dup15q syndrome.

Date: 2021
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DOI: 10.1038/s41467-021-24359-3

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