Fast holographic scattering compensation for deep tissue biological imaging

Molly A. May (), Nicolas Barré, Kai K. Kummer, Michaela Kress, Monika Ritsch-Marte and Alexander Jesacher
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Molly A. May: Medical University of Innsbruck
Nicolas Barré: Medical University of Innsbruck
Kai K. Kummer: Medical University of Innsbruck
Michaela Kress: Medical University of Innsbruck
Monika Ritsch-Marte: Medical University of Innsbruck
Alexander Jesacher: Medical University of Innsbruck

Nature Communications, 2021, vol. 12, issue 1, 1-8

Abstract: Abstract Scattering in biological tissues is a major barrier for in vivo optical imaging of all but the most superficial structures. Progress toward overcoming the distortions caused by scattering in turbid media has been made by shaping the excitation wavefront to redirect power into a single point in the imaging plane. However, fast, non-invasive determination of the required wavefront compensation remains challenging. Here, we introduce a quickly converging algorithm for non-invasive scattering compensation, termed DASH, in which holographic phase stepping interferometry enables new phase information to be updated after each measurement. This leads to rapid improvement of the wavefront correction, forming a focus after just one measurement iteration and achieving an order of magnitude higher signal enhancement at this stage than the previous state-of-the-art. Using DASH, we demonstrate two-photon fluorescence imaging of microglia cells in highly turbid mouse hippocampal tissue down to a depth of 530 μm.

Date: 2021
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DOI: 10.1038/s41467-021-24666-9

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