Reverse optogenetics of G protein signaling by zebrafish non-visual opsin Opn7b for synchronization of neuronal networks

Raziye Karapinar, Jan Claudius Schwitalla, Dennis Eickelbeck, Johanna Pakusch, Brix Mücher, Michelle Grömmke, Tatjana Surdin, Thomas Knöpfel, Melanie D. Mark (), Ida Siveke and Stefan Herlitze ()
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Raziye Karapinar: Ruhr-University Bochum
Jan Claudius Schwitalla: Ruhr-University Bochum
Dennis Eickelbeck: Ruhr-University Bochum
Johanna Pakusch: Ruhr-University Bochum
Brix Mücher: Ruhr-University Bochum
Michelle Grömmke: Ruhr-University Bochum
Tatjana Surdin: Ruhr-University Bochum
Thomas Knöpfel: Imperial College London
Melanie D. Mark: Ruhr-University Bochum
Ida Siveke: Ruhr-University Bochum
Stefan Herlitze: Ruhr-University Bochum

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract Opn7b is a non-visual G protein-coupled receptor expressed in zebrafish. Here we find that Opn7b expressed in HEK cells constitutively activates the Gi/o pathway and illumination with blue/green light inactivates G protein-coupled inwardly rectifying potassium channels. This suggests that light acts as an inverse agonist for Opn7b and can be used as an optogenetic tool to inhibit neuronal networks in the dark and interrupt constitutive inhibition in the light. Consistent with this prediction, illumination of recombinant expressed Opn7b in cortical pyramidal cells results in increased neuronal activity. In awake mice, light stimulation of Opn7b expressed in pyramidal cells of somatosensory cortex reliably induces generalized epileptiform activity within a short (

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24718-0

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DOI: 10.1038/s41467-021-24718-0

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