BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons

Johannes Vierock, Silvia Rodriguez-Rozada, Alexander Dieter, Florian Pieper, Ruth Sims, Federico Tenedini, Amelie C. F. Bergs, Imane Bendifallah, Fangmin Zhou, Nadja Zeitzschel, Joachim Ahlbeck, Sandra Augustin, Kathrin Sauter, Eirini Papagiakoumou, Alexander Gottschalk, Peter Soba, Valentina Emiliani, Andreas K. Engel, Peter Hegemann and J. Simon Wiegert ()
Additional contact information
Johannes Vierock: Humboldt University Berlin
Silvia Rodriguez-Rozada: University Medical Center Hamburg-Eppendorf
Alexander Dieter: University Medical Center Hamburg-Eppendorf
Florian Pieper: University Medical Center Hamburg-Eppendorf
Ruth Sims: Sorbonne Université, INSERM, CNRS, Institut de la Vision
Federico Tenedini: University Medical Center Hamburg-Eppendorf
Amelie C. F. Bergs: Goethe University
Imane Bendifallah: Sorbonne Université, INSERM, CNRS, Institut de la Vision
Fangmin Zhou: University Medical Center Hamburg-Eppendorf
Nadja Zeitzschel: Goethe University
Joachim Ahlbeck: University Medical Center Hamburg-Eppendorf
Sandra Augustin: Humboldt University Berlin
Kathrin Sauter: University Medical Center Hamburg-Eppendorf
Eirini Papagiakoumou: Sorbonne Université, INSERM, CNRS, Institut de la Vision
Alexander Gottschalk: Goethe University
Peter Soba: University Medical Center Hamburg-Eppendorf
Valentina Emiliani: Sorbonne Université, INSERM, CNRS, Institut de la Vision
Andreas K. Engel: University Medical Center Hamburg-Eppendorf
Peter Hegemann: Humboldt University Berlin
J. Simon Wiegert: University Medical Center Hamburg-Eppendorf

Nature Communications, 2021, vol. 12, issue 1, 1-20

Abstract: Abstract Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control of neuronal activity allowing both excitation and inhibition of the same neurons in a single experiment is desired. This requires low spectral overlap between the excitatory and inhibitory opsin, matched photocurrent amplitudes and a fixed expression ratio. Moreover, independent activation of two distinct neuronal populations with different optogenetic actuators is still challenging due to blue-light sensitivity of all opsins. Here we report BiPOLES, an optogenetic tool for potent neuronal excitation and inhibition with light of two different wavelengths. BiPOLES enables sensitive, reliable dual-color neuronal spiking and silencing with single- or two-photon excitation, optical tuning of the membrane voltage, and independent optogenetic control of two neuronal populations using a second, blue-light sensitive opsin. The utility of BiPOLES is demonstrated in worms, flies, mice and ferrets.

Date: 2021
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DOI: 10.1038/s41467-021-24759-5

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