Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids

Ann-Na Cho, Yoonhee Jin, Yeonjoo An, Jin Kim, Yi Sun Choi, Jung Seung Lee, Junghoon Kim, Won-Young Choi, Dong-Jun Koo, Weonjin Yu, Gyeong-Eon Chang, Dong-Yoon Kim, Sung-Hyun Jo, Jihun Kim, Sung-Yon Kim, Yun-Gon Kim, Ju Young Kim, Nakwon Choi, Eunji Cheong, Young-Joon Kim, Hyunsoo Shawn Je, Hoon-Chul Kang and Seung-Woo Cho ()
Additional contact information
Ann-Na Cho: Yonsei University
Yoonhee Jin: Yonsei University
Yeonjoo An: Yonsei University
Jin Kim: Yonsei University
Yi Sun Choi: Yonsei University
Jung Seung Lee: Yonsei University
Junghoon Kim: Yonsei University
Won-Young Choi: Yonsei University
Dong-Jun Koo: Seoul National University
Weonjin Yu: Duke-NUS Medical School
Gyeong-Eon Chang: Yonsei University
Dong-Yoon Kim: Seoul National University
Sung-Hyun Jo: Soongsil University
Jihun Kim: Yonsei University College of Medicine
Sung-Yon Kim: Seoul National University
Yun-Gon Kim: Soongsil University
Ju Young Kim: Kangwon National University
Nakwon Choi: Korea Institute of Science and Technology (KIST)
Eunji Cheong: Yonsei University
Young-Joon Kim: Yonsei University
Hyunsoo Shawn Je: Duke-NUS Medical School
Hoon-Chul Kang: Yonsei University College of Medicine
Seung-Woo Cho: Yonsei University

Nature Communications, 2021, vol. 12, issue 1, 1-23

Abstract: Abstract Brain organoids derived from human pluripotent stem cells provide a highly valuable in vitro model to recapitulate human brain development and neurological diseases. However, the current systems for brain organoid culture require further improvement for the reliable production of high-quality organoids. Here, we demonstrate two engineering elements to improve human brain organoid culture, (1) a human brain extracellular matrix to provide brain-specific cues and (2) a microfluidic device with periodic flow to improve the survival and reduce the variability of organoids. A three-dimensional culture modified with brain extracellular matrix significantly enhanced neurogenesis in developing brain organoids from human induced pluripotent stem cells. Cortical layer development, volumetric augmentation, and electrophysiological function of human brain organoids were further improved in a reproducible manner by dynamic culture in microfluidic chamber devices. Our engineering concept of reconstituting brain-mimetic microenvironments facilitates the development of a reliable culture platform for brain organoids, enabling effective modeling and drug development for human brain diseases.

Date: 2021
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-021-24775-5 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24775-5

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-021-24775-5

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().