Label-free imaging for quality control of cardiomyocyte differentiation

Tongcheng Qian (), Tiffany M. Heaster, Angela R. Houghtaling, Kexin Sun, Kayvan Samimi and Melissa C. Skala ()
Additional contact information
Tongcheng Qian: Morgridge Institute for Research
Tiffany M. Heaster: Morgridge Institute for Research
Angela R. Houghtaling: Morgridge Institute for Research
Kexin Sun: Morgridge Institute for Research
Kayvan Samimi: Morgridge Institute for Research
Melissa C. Skala: Morgridge Institute for Research

Nature Communications, 2021, vol. 12, issue 1, 1-11

Abstract: Abstract Human pluripotent stem cell (hPSC)-derived cardiomyocytes provide a promising regenerative cell therapy for cardiovascular patients and an important model system to accelerate drug discovery. However, cost-effective and time-efficient platforms must be developed to evaluate the quality of hPSC-derived cardiomyocytes during biomanufacturing. Here, we develop a non-invasive label-free live cell imaging platform to predict the efficiency of hPSC differentiation into cardiomyocytes. Autofluorescence imaging of metabolic co-enzymes is performed under varying differentiation conditions (cell density, concentration of Wnt signaling activator) across five hPSC lines. Live cell autofluorescence imaging and multivariate classification models provide high accuracy to separate low (

Date: 2021
References: Add references at CitEc
Citations:

Downloads: (external link)
https://www.nature.com/articles/s41467-021-24868-1 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24868-1

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-021-24868-1

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().