Variant PCGF1-PRC1 links PRC2 recruitment with differentiation-associated transcriptional inactivation at target genes

Hiroki Sugishita, Takashi Kondo, Shinsuke Ito, Manabu Nakayama, Nayuta Yakushiji-Kaminatsui, Eiryo Kawakami, Yoko Koseki, Yasuhide Ohinata, Jafar Sharif, Mio Harachi, Neil P. Blackledge, Robert J. Klose and Haruhiko Koseki ()
Additional contact information
Hiroki Sugishita: RIKEN Center for Integrative Medical Sciences
Takashi Kondo: RIKEN Center for Integrative Medical Sciences
Shinsuke Ito: RIKEN Center for Integrative Medical Sciences
Manabu Nakayama: Kazusa DNA Research Institute
Nayuta Yakushiji-Kaminatsui: RIKEN Center for Integrative Medical Sciences
Eiryo Kawakami: Chiba University
Yoko Koseki: RIKEN Center for Integrative Medical Sciences
Yasuhide Ohinata: RIKEN Center for Integrative Medical Sciences
Jafar Sharif: RIKEN Center for Integrative Medical Sciences
Mio Harachi: RIKEN Center for Integrative Medical Sciences
Neil P. Blackledge: University of Oxford
Robert J. Klose: University of Oxford
Haruhiko Koseki: RIKEN Center for Integrative Medical Sciences

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract Polycomb repressive complexes-1 and -2 (PRC1 and 2) silence developmental genes in a spatiotemporal manner during embryogenesis. How Polycomb group (PcG) proteins orchestrate down-regulation of target genes upon differentiation, however, remains elusive. Here, by differentiating embryonic stem cells into embryoid bodies, we reveal a crucial role for the PCGF1-containing variant PRC1 complex (PCGF1-PRC1) to mediate differentiation-associated down-regulation of a group of genes. Upon differentiation cues, transcription is down-regulated at these genes, in association with PCGF1-PRC1-mediated deposition of histone H2AK119 mono-ubiquitination (H2AK119ub1) and PRC2 recruitment. In the absence of PCGF1-PRC1, both H2AK119ub1 deposition and PRC2 recruitment are disrupted, leading to aberrant expression of target genes. PCGF1-PRC1 is, therefore, required for initiation and consolidation of PcG-mediated gene repression during differentiation.

Date: 2021
References: Add references at CitEc
Citations: View citations in EconPapers (2)

Downloads: (external link)
https://www.nature.com/articles/s41467-021-24894-z Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24894-z

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-021-24894-z

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().