Non-invasive hydrodynamic imaging in plant roots at cellular resolution

Flavius C. Pascut (), Valentin Couvreur (), Daniela Dietrich, Nicky Leftley, Guilhem Reyt, Yann Boursiac, Monica Calvo-Polanco, Ilda Casimiro, Christophe Maurel, David E. Salt, Xavier Draye, Darren M. Wells, Malcolm J. Bennett and Kevin F. Webb
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Flavius C. Pascut: University of Nottingham
Valentin Couvreur: Université catholique de Louvain
Daniela Dietrich: University of Bristol
Nicky Leftley: University of Nottingham
Guilhem Reyt: University of Nottingham
Yann Boursiac: BPMP, Univ Montpellier, CNRS, INRAE, Institut Agro
Monica Calvo-Polanco: BPMP, Univ Montpellier, CNRS, INRAE, Institut Agro
Ilda Casimiro: Universidad de Extremadura, Facultad de Ciencias
Christophe Maurel: BPMP, Univ Montpellier, CNRS, INRAE, Institut Agro
David E. Salt: University of Nottingham
Xavier Draye: Université catholique de Louvain
Darren M. Wells: University of Nottingham
Malcolm J. Bennett: University of Nottingham
Kevin F. Webb: University of Nottingham

Nature Communications, 2021, vol. 12, issue 1, 1-7

Abstract: Abstract A key impediment to studying water-related mechanisms in plants is the inability to non-invasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelling, can achieve this goal - monitoring hydrodynamics within living root tissues at cell- and sub-second-scale resolutions. Raman imaging of water-transporting xylem vessels in Arabidopsis thaliana mutant roots reveals faster xylem water transport in endodermal diffusion barrier mutants. Furthermore, transverse line scans across the root suggest water transported via the root xylem does not re-enter outer root tissues nor the surrounding soil when en-route to shoot tissues if endodermal diffusion barriers are intact, thereby separating ‘two water worlds’.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-24913-z

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DOI: 10.1038/s41467-021-24913-z

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