The developing mouse coronal suture at single-cell resolution

D’Juan T. Farmer, Hana Mlcochova, Yan Zhou, Nils Koelling, Guanlin Wang, Neil Ashley, Helena Bugacov, Hung-Jhen Chen, Riana Parvez, Kuo-Chang Tseng, Amy E. Merrill, Robert E. Maxson, Andrew O. M. Wilkie, J. Gage Crump () and Stephen R. F. Twigg ()
Additional contact information
D’Juan T. Farmer: University of Southern California
Hana Mlcochova: University of Oxford
Yan Zhou: University of Oxford
Nils Koelling: University of Oxford
Guanlin Wang: University of Oxford
Neil Ashley: University of Oxford
Helena Bugacov: University of Southern California
Hung-Jhen Chen: University of Southern California
Riana Parvez: University of Southern California
Kuo-Chang Tseng: University of Southern California
Amy E. Merrill: University of Southern California
Robert E. Maxson: University of Southern California
Andrew O. M. Wilkie: University of Oxford
J. Gage Crump: University of Southern California
Stephen R. F. Twigg: University of Oxford

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract Sutures separate the flat bones of the skull and enable coordinated growth of the brain and overlying cranium. The coronal suture is most commonly fused in monogenic craniosynostosis, yet the unique aspects of its development remain incompletely understood. To uncover the cellular diversity within the murine embryonic coronal suture, we generated single-cell transcriptomes and performed extensive expression validation. We find distinct pre-osteoblast signatures between the bone fronts and periosteum, a ligament-like population above the suture that persists into adulthood, and a chondrogenic-like population in the dura mater underlying the suture. Lineage tracing reveals an embryonic Six2+ osteoprogenitor population that contributes to the postnatal suture mesenchyme, with these progenitors being preferentially affected in a Twist1+/−; Tcf12+/− mouse model of Saethre-Chotzen Syndrome. This single-cell atlas provides a resource for understanding the development of the coronal suture and the mechanisms for its loss in craniosynostosis.

Date: 2021
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DOI: 10.1038/s41467-021-24917-9

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