Quantifying nanodiamonds biodistribution in whole cells with correlative iono-nanoscopy

Zhaohong Mi, Ce-Belle Chen, Hong Qi Tan, Yanxin Dou, Chengyuan Yang, Shuvan Prashant Turaga, Minqin Ren, Saumitra K. Vajandar, Gin Hao Yuen, Thomas Osipowicz, Frank Watt () and Andrew A. Bettiol ()
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Zhaohong Mi: National University of Singapore
Ce-Belle Chen: National University of Singapore
Hong Qi Tan: National University of Singapore
Yanxin Dou: National University of Singapore
Chengyuan Yang: National University of Singapore
Shuvan Prashant Turaga: National University of Singapore
Minqin Ren: National University of Singapore
Saumitra K. Vajandar: National University of Singapore
Gin Hao Yuen: National University of Singapore
Thomas Osipowicz: National University of Singapore
Frank Watt: National University of Singapore
Andrew A. Bettiol: National University of Singapore

Nature Communications, 2021, vol. 12, issue 1, 1-9

Abstract: Abstract Correlative imaging and quantification of intracellular nanoparticles with the underlying ultrastructure is crucial for understanding cell-nanoparticle interactions in biological research. However, correlative nanoscale imaging of whole cells still remains a daunting challenge. Here, we report a straightforward nanoscopic approach for whole-cell correlative imaging, by simultaneous ionoluminescence and ultrastructure mapping implemented with a highly focused beam of alpha particles. We demonstrate that fluorescent nanodiamonds exhibit fast, ultrabright and stable emission upon excitation by alpha particles. Thus, by using fluorescent nanodiamonds as imaging probes, our approach enables quantification and correlative localization of single nanodiamonds within a whole cell at sub-30 nm resolution. As an application example, we show that our approach, together with Monte Carlo simulations and radiobiological experiments, can be employed to provide unique insights into the mechanisms of nanodiamond radiosensitization at the single whole-cell level. These findings may benefit clinical studies of radio-enhancement effects by nanoparticles in charged-particle cancer therapy.

Date: 2021
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DOI: 10.1038/s41467-021-25004-9

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