The regulatory impact of RNA-binding proteins on microRNA targeting

Sukjun Kim, Soyoung Kim, Hee Ryung Chang, Doyeon Kim, Junehee Park, Narae Son, Joori Park, Minhyuk Yoon, Gwangung Chae, Young-Kook Kim, V. Narry Kim, Yoon Ki Kim, Jin-Wu Nam, Chanseok Shin () and Daehyun Baek ()
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Sukjun Kim: Seoul National University
Soyoung Kim: Seoul National University
Hee Ryung Chang: Seoul National University
Doyeon Kim: Seoul National University
Junehee Park: Seoul National University
Narae Son: Seoul National University
Joori Park: Korea University
Minhyuk Yoon: Seoul National University
Gwangung Chae: Seoul National University
Young-Kook Kim: Chonnam National University Medical School
V. Narry Kim: Seoul National University
Yoon Ki Kim: Korea University
Jin-Wu Nam: Hanyang University
Chanseok Shin: Seoul National University
Daehyun Baek: Seoul National University

Nature Communications, 2021, vol. 12, issue 1, 1-15

Abstract: Abstract Argonaute is the primary mediator of metazoan miRNA targeting (MT). Among the currently identified >1,500 human RNA-binding proteins (RBPs), there are only a handful of RBPs known to enhance MT and several others reported to suppress MT, leaving the global impact of RBPs on MT elusive. In this study, we have systematically analyzed transcriptome-wide binding sites for 150 human RBPs and evaluated the quantitative effect of individual RBPs on MT efficacy. In contrast to previous studies, we show that most RBPs significantly affect MT and that all of those MT-regulating RBPs function as MT enhancers rather than suppressors, by making the local secondary structure of the target site accessible to Argonaute. Our findings illuminate the unappreciated regulatory impact of human RBPs on MT, and as these RBPs may play key roles in the gene regulatory network governed by metazoan miRNAs, MT should be understood in the context of co-regulating RBPs.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25078-5

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DOI: 10.1038/s41467-021-25078-5

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