Amnion signals are essential for mesoderm formation in primates

Ran Yang, Alexander Goedel, Yu Kang, Chenyang Si, Chu Chu, Yi Zheng, Zhenzhen Chen, Peter J. Gruber, Yao Xiao, Chikai Zhou, Nevin Witman, Elif Eroglu, Chuen-Yan Leung, Yongchang Chen, Jianping Fu, Weizhi Ji (), Fredrik Lanner (), Yuyu Niu () and Kenneth R. Chien ()
Additional contact information
Ran Yang: Karolinska Institutet
Alexander Goedel: Karolinska Institutet
Yu Kang: Kunming University of Science and Technology
Chenyang Si: Kunming University of Science and Technology
Chu Chu: Kunming University of Science and Technology
Yi Zheng: University of Michigan
Zhenzhen Chen: Kunming University of Science and Technology
Peter J. Gruber: Karolinska Institutet
Yao Xiao: Karolinska Institutet
Chikai Zhou: Karolinska Institutet
Nevin Witman: Karolinska Institutet
Elif Eroglu: Karolinska Institutet
Chuen-Yan Leung: Karolinska Institutet
Yongchang Chen: Kunming University of Science and Technology
Jianping Fu: University of Michigan
Weizhi Ji: Kunming University of Science and Technology
Fredrik Lanner: Karolinska Institutet
Yuyu Niu: Kunming University of Science and Technology
Kenneth R. Chien: Karolinska Institutet

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract Embryonic development is largely conserved among mammals. However, certain genes show divergent functions. By generating a transcriptional atlas containing >30,000 cells from post-implantation non-human primate embryos, we uncover that ISL1, a gene with a well-established role in cardiogenesis, controls a gene regulatory network in primate amnion. CRISPR/Cas9-targeting of ISL1 results in non-human primate embryos which do not yield viable offspring, demonstrating that ISL1 is critically required in primate embryogenesis. On a cellular level, mutant ISL1 embryos display a failure in mesoderm formation due to reduced BMP4 signaling from the amnion. Via loss of function and rescue studies in human embryonic stem cells we confirm a similar role of ISL1 in human in vitro derived amnion. This study highlights the importance of the amnion as a signaling center during primate mesoderm formation and demonstrates the potential of in vitro primate model systems to dissect the genetics of early human embryonic development.

Date: 2021
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DOI: 10.1038/s41467-021-25186-2

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