Complete and efficient conversion of plant cell wall hemicellulose into high-value bioproducts by engineered yeast

Liang Sun, Jae Won Lee, Sangdo Yook, Stephan Lane, Ziqiao Sun, Soo Rin Kim and Yong-Su Jin ()
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Liang Sun: University of Illinois at Urbana-Champaign
Jae Won Lee: University of Illinois at Urbana-Champaign
Sangdo Yook: University of Illinois at Urbana-Champaign
Stephan Lane: University of Illinois at Urbana-Champaign
Ziqiao Sun: University of Illinois at Urbana-Champaign
Soo Rin Kim: Kyungpook National University
Yong-Su Jin: University of Illinois at Urbana-Champaign

Nature Communications, 2021, vol. 12, issue 1, 1-9

Abstract: Abstract Plant cell wall hydrolysates contain not only sugars but also substantial amounts of acetate, a fermentation inhibitor that hinders bioconversion of lignocellulose. Despite the toxic and non-consumable nature of acetate during glucose metabolism, we demonstrate that acetate can be rapidly co-consumed with xylose by engineered Saccharomyces cerevisiae. The co-consumption leads to a metabolic re-configuration that boosts the synthesis of acetyl-CoA derived bioproducts, including triacetic acid lactone (TAL) and vitamin A, in engineered strains. Notably, by co-feeding xylose and acetate, an enginered strain produces 23.91 g/L TAL with a productivity of 0.29 g/L/h in bioreactor fermentation. This strain also completely converts a hemicellulose hydrolysate of switchgrass into 3.55 g/L TAL. These findings establish a versatile strategy that not only transforms an inhibitor into a valuable substrate but also expands the capacity of acetyl-CoA supply in S. cerevisiae for efficient bioconversion of cellulosic biomass.

Date: 2021
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DOI: 10.1038/s41467-021-25241-y

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