Structural basis for ALK2/BMPR2 receptor complex signaling through kinase domain oligomerization

Christopher Agnew, Pelin Ayaz, Risa Kashima, Hanna S. Loving, Prajakta Ghatpande, Jennifer E. Kung, Eric S. Underbakke (), Yibing Shan (), David E. Shaw (), Akiko Hata and Natalia Jura ()
Additional contact information
Christopher Agnew: University of California San Francisco
Pelin Ayaz: D. E. Shaw Research
Risa Kashima: University of California San Francisco
Hanna S. Loving: Iowa State University
Prajakta Ghatpande: University of California San Francisco
Jennifer E. Kung: University of California San Francisco
Eric S. Underbakke: Iowa State University
Yibing Shan: D. E. Shaw Research
David E. Shaw: D. E. Shaw Research
Akiko Hata: University of California San Francisco
Natalia Jura: University of California San Francisco

Nature Communications, 2021, vol. 12, issue 1, 1-17

Abstract: Abstract Upon ligand binding, bone morphogenetic protein (BMP) receptors form active tetrameric complexes, comprised of two type I and two type II receptors, which then transmit signals to SMAD proteins. The link between receptor tetramerization and the mechanism of kinase activation, however, has not been elucidated. Here, using hydrogen deuterium exchange mass spectrometry (HDX-MS), small angle X-ray scattering (SAXS) and molecular dynamics (MD) simulations, combined with analysis of SMAD signaling, we show that the kinase domain of the type I receptor ALK2 and type II receptor BMPR2 form a heterodimeric complex via their C-terminal lobes. Formation of this dimer is essential for ligand-induced receptor signaling and is targeted by mutations in BMPR2 in patients with pulmonary arterial hypertension (PAH). We further show that the type I/type II kinase domain heterodimer serves as the scaffold for assembly of the active tetrameric receptor complexes to enable phosphorylation of the GS domain and activation of SMADs.

Date: 2021
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DOI: 10.1038/s41467-021-25248-5

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