DMRT1-mediated reprogramming drives development of cancer resembling human germ cell tumors with features of totipotency

Jumpei Taguchi, Hirofumi Shibata, Mio Kabata, Masaki Kato, Kei Fukuda, Akito Tanaka, Sho Ohta, Tomoyo Ukai, Kanae Mitsunaga, Yosuke Yamada, So I Nagaoka, Sho Yamazawa, Kotaro Ohnishi, Knut Woltjen, Tetsuo Ushiku, Manabu Ozawa, Mitinori Saitou, Yoichi Shinkai, Takuya Yamamoto and Yasuhiro Yamada ()
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Jumpei Taguchi: The University of Tokyo, Minoto-ku
Hirofumi Shibata: Kyoto University, Sakyo-ku
Mio Kabata: Kyoto University, Sakyo-ku
Masaki Kato: Cellular Memory Laboratory, RIKEN Cluster for Pioneering Research, Wako-shi
Kei Fukuda: Cellular Memory Laboratory, RIKEN Cluster for Pioneering Research, Wako-shi
Akito Tanaka: Kyoto University, Sakyo-ku
Sho Ohta: The University of Tokyo, Minoto-ku
Tomoyo Ukai: The University of Tokyo, Minoto-ku
Kanae Mitsunaga: Kyoto University, Sakyo-ku
Yosuke Yamada: Kyoto University, Sakyo-ku
So I Nagaoka: Kyoto University, Yoshida-Konoe-cho, Sakyo-ku
Sho Yamazawa: The University of Tokyo
Kotaro Ohnishi: Kyoto University, Sakyo-ku
Knut Woltjen: Kyoto University, Sakyo-ku
Tetsuo Ushiku: The University of Tokyo
Manabu Ozawa: The University of Tokyo
Mitinori Saitou: Kyoto University, Sakyo-ku
Yoichi Shinkai: Cellular Memory Laboratory, RIKEN Cluster for Pioneering Research, Wako-shi
Takuya Yamamoto: Kyoto University, Sakyo-ku
Yasuhiro Yamada: The University of Tokyo, Minoto-ku

Nature Communications, 2021, vol. 12, issue 1, 1-18

Abstract: Abstract In vivo reprogramming provokes a wide range of cell fate conversion. Here, we discover that in vivo induction of higher levels of OSKM in mouse somatic cells leads to increased expression of primordial germ cell (PGC)-related genes and provokes genome-wide erasure of genomic imprinting, which takes place exclusively in PGCs. Moreover, the in vivo OSKM reprogramming results in development of cancer that resembles human germ cell tumors. Like a subgroup of germ cell tumors, propagated tumor cells can differentiate into trophoblasts. Moreover, these tumor cells give rise to induced pluripotent stem cells (iPSCs) with expanded differentiation potential into trophoblasts. Remarkably, the tumor-derived iPSCs are able to contribute to non-neoplastic somatic cells in adult mice. Mechanistically, DMRT1, which is expressed in PGCs, drives the reprogramming and propagation of the tumor cells in vivo. Furthermore, the DMRT1-related epigenetic landscape is associated with trophoblast competence of the reprogrammed cells and provides a therapeutic target for germ cell tumors. These results reveal an unappreciated route for somatic cell reprogramming and underscore the impact of reprogramming in development of germ cell tumors.

Date: 2021
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DOI: 10.1038/s41467-021-25249-4

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