RNF168-mediated localization of BARD1 recruits the BRCA1-PALB2 complex to DNA damage

John J. Krais, Yifan Wang, Pooja Patel, Jayati Basu, Andrea J. Bernhardy and Neil Johnson ()
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John J. Krais: Molecular Therapeutics Program, Fox Chase Cancer Center
Yifan Wang: Molecular Therapeutics Program, Fox Chase Cancer Center
Pooja Patel: Molecular Therapeutics Program, Fox Chase Cancer Center
Jayati Basu: Blood Cell Development and Function Program, Fox Chase Cancer Center
Andrea J. Bernhardy: Molecular Therapeutics Program, Fox Chase Cancer Center
Neil Johnson: Molecular Therapeutics Program, Fox Chase Cancer Center

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract DNA damage prompts a diverse range of alterations to the chromatin landscape. The RNF168 E3 ubiquitin ligase catalyzes the mono-ubiquitination of histone H2A at lysine (K)13/15 (mUb-H2A), forming a binding module for DNA repair proteins. BRCA1 promotes homologous recombination (HR), in part, through its interaction with PALB2, and the formation of a larger BRCA1-PALB2-BRCA2-RAD51 (BRCA1-P) complex. The mechanism by which BRCA1-P is recruited to chromatin surrounding DNA breaks is unclear. In this study, we reveal that an RNF168-governed signaling pathway is responsible for localizing the BRCA1-P complex to DNA damage. Using mice harboring a Brca1CC (coiled coil) mutation that blocks the Brca1-Palb2 interaction, we uncovered an epistatic relationship between Rnf168− and Brca1CC alleles, which disrupted development, and reduced the efficiency of Palb2-Rad51 localization. Mechanistically, we show that RNF168-generated mUb-H2A recruits BARD1 through a BRCT domain ubiquitin-dependent recruitment motif (BUDR). Subsequently, BARD1-BRCA1 accumulate PALB2-RAD51 at DNA breaks via the CC domain-mediated BRCA1-PALB2 interaction. Together, these findings establish a series of molecular interactions that connect the DNA damage signaling and HR repair machinery.

Date: 2021
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DOI: 10.1038/s41467-021-25346-4

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