In situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli

Philipp S. Erdmann (), Zhen Hou, Sven Klumpe, Sagar Khavnekar, Florian Beck, Florian Wilfling, Jürgen M. Plitzko and Wolfgang Baumeister ()
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Philipp S. Erdmann: Max Planck Institute of Biochemistry
Zhen Hou: Max Planck Institute of Biochemistry
Sven Klumpe: Max Planck Institute of Biochemistry
Sagar Khavnekar: Max Planck Institute of Biochemistry
Florian Beck: Max Planck Institute of Biochemistry
Florian Wilfling: Max Planck Institute of Biochemistry
Jürgen M. Plitzko: Max Planck Institute of Biochemistry
Wolfgang Baumeister: Max Planck Institute of Biochemistry

Nature Communications, 2021, vol. 12, issue 1, 1-9

Abstract: Abstract Ribosomes comprise a large (LSU) and a small subunit (SSU) which are synthesized independently in the nucleolus before being exported into the cytoplasm, where they assemble into functional ribosomes. Individual maturation steps have been analyzed in detail using biochemical methods, light microscopy and conventional electron microscopy (EM). In recent years, single particle analysis (SPA) has yielded molecular resolution structures of several pre-ribosomal intermediates. It falls short, however, of revealing the spatiotemporal sequence of ribosome biogenesis in the cellular context. Here, we present our study on native nucleoli in Chlamydomonas reinhardtii, in which we follow the formation of LSU and SSU precursors by in situ cryo-electron tomography (cryo-ET) and subtomogram averaging (STA). By combining both positional and molecular data, we reveal gradients of ribosome maturation within the granular component (GC), offering a new perspective on how the liquid-liquid-phase separation of the nucleolus supports ribosome biogenesis.

Date: 2021
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DOI: 10.1038/s41467-021-25413-w

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