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Processive dynamics of the usher assembly platform during uropathogenic Escherichia coli P pilus biogenesis

Minge Du, Zuanning Yuan, Glenn T. Werneburg, Nadine S. Henderson, Hemil Chauhan, Amanda Kovach, Gongpu Zhao, Jessica Johl, Huilin Li () and David G. Thanassi ()
Additional contact information
Minge Du: Van Andel Institute
Zuanning Yuan: Van Andel Institute
Glenn T. Werneburg: Stony Brook University, Stony Brook
Nadine S. Henderson: Stony Brook University, Stony Brook
Hemil Chauhan: Stony Brook University, Stony Brook
Amanda Kovach: Van Andel Institute
Gongpu Zhao: Van Andel Institute
Jessica Johl: Stony Brook University, Stony Brook
Huilin Li: Van Andel Institute
David G. Thanassi: Stony Brook University, Stony Brook

Nature Communications, 2021, vol. 12, issue 1, 1-9

Abstract: Abstract Uropathogenic Escherichia coli assemble surface structures termed pili or fimbriae to initiate infection of the urinary tract. P pili facilitate bacterial colonization of the kidney and pyelonephritis. P pili are assembled through the conserved chaperone-usher pathway. Much of the structural and functional understanding of the chaperone-usher pathway has been gained through investigations of type 1 pili, which promote binding to the bladder and cystitis. In contrast, the structural basis for P pilus biogenesis at the usher has remained elusive. This is in part due to the flexible and variable-length P pilus tip fiber, creating structural heterogeneity, and difficulties isolating stable P pilus assembly intermediates. Here, we circumvent these hindrances and determine cryo-electron microscopy structures of the activated PapC usher in the process of secreting two- and three-subunit P pilus assembly intermediates, revealing processive steps in P pilus biogenesis and capturing new conformational dynamics of the usher assembly machine.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25522-6

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DOI: 10.1038/s41467-021-25522-6

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