Brown adipose tissue monocytes support tissue expansion

Alexandre Gallerand, Marion I. Stunault, Johanna Merlin, Hannah P. Luehmann, Deborah H. Sultan, Maria M. Firulyova, Virginie Magnone, Narges Khedher, Antoine Jalil, Bastien Dolfi, Alexia Castiglione, Adelie Dumont, Marion Ayrault, Nathalie Vaillant, Jérôme Gilleron, Pascal Barbry, David Dombrowicz, Matthias Mack, David Masson, Thomas Bertero, Burkhard Becher, Jesse W. Williams, Konstantin Zaitsev, Yongjian Liu, Rodolphe R. Guinamard, Laurent Yvan-Charvet and Stoyan Ivanov ()
Additional contact information
Alexandre Gallerand: Université Côte d’Azur, INSERM, C3M
Marion I. Stunault: Université Côte d’Azur, INSERM, C3M
Johanna Merlin: Université Côte d’Azur, INSERM, C3M
Hannah P. Luehmann: Washington University School of Medicine
Deborah H. Sultan: Washington University School of Medicine
Maria M. Firulyova: ITMO University
Virginie Magnone: Université Côte d’Azur, CNRS, IPMC
Narges Khedher: Université Côte d’Azur, INSERM, C3M
Antoine Jalil: Université Bourgogne Franche-Comté, LNC UMR1231
Bastien Dolfi: Université Côte d’Azur, INSERM, C3M
Alexia Castiglione: Université Côte d’Azur, INSERM, C3M
Adelie Dumont: Université Côte d’Azur, INSERM, C3M
Marion Ayrault: Université Côte d’Azur, INSERM, C3M
Nathalie Vaillant: Université Côte d’Azur, INSERM, C3M
Jérôme Gilleron: Université Côte d’Azur, INSERM, C3M
Pascal Barbry: Université Côte d’Azur, CNRS, IPMC
David Dombrowicz: Univ.Lille, Inserm, CHU Lille, Institut Pasteur de Lille, U1011-EGID
Matthias Mack: University Hospital Regensburg
David Masson: Université Bourgogne Franche-Comté, LNC UMR1231
Thomas Bertero: Université Côte d’Azur, CNRS, IPMC
Burkhard Becher: Institute of Experimental Immunology, University of Zürich
Jesse W. Williams: University of Minnesota Medical School
Konstantin Zaitsev: ITMO University
Yongjian Liu: Washington University School of Medicine
Rodolphe R. Guinamard: Université Côte d’Azur, INSERM, C3M
Laurent Yvan-Charvet: Université Côte d’Azur, INSERM, C3M
Stoyan Ivanov: Université Côte d’Azur, INSERM, C3M

Nature Communications, 2021, vol. 12, issue 1, 1-13

Abstract: Abstract Monocytes are part of the mononuclear phagocytic system. Monocytes play a central role during inflammatory conditions and a better understanding of their dynamics might open therapeutic opportunities. In the present study, we focused on the characterization and impact of monocytes on brown adipose tissue (BAT) functions during tissue remodeling. Single-cell RNA sequencing analysis of BAT immune cells uncovered a large diversity in monocyte and macrophage populations. Fate-mapping experiments demonstrated that the BAT macrophage pool requires constant replenishment from monocytes. Using a genetic model of BAT expansion, we found that brown fat monocyte numbers were selectively increased in this scenario. This observation was confirmed using a CCR2-binding radiotracer and positron emission tomography. Importantly, in line with their tissue recruitment, blood monocyte counts were decreased while bone marrow hematopoiesis was not affected. Monocyte depletion prevented brown adipose tissue expansion and altered its architecture. Podoplanin engagement is strictly required for BAT expansion. Together, these data redefine the diversity of immune cells in the BAT and emphasize the role of monocyte recruitment for tissue remodeling.

Date: 2021
References: Add references at CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-021-25616-1 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-25616-1

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-021-25616-1

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().