Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Myungjae Song, Jung Min Lim, Seonwoo Min, Jeong-Seok Oh, Dong Young Kim, Jae-Sung Woo, Hiroshi Nishimasu, Sung-Rae Cho, Sungroh Yoon and Hyongbum Henry Kim ()
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Myungjae Song: Yonsei University College of Medicine
Jung Min Lim: Yonsei University College of Medicine
Seonwoo Min: Seoul National University
Jeong-Seok Oh: Korea University
Dong Young Kim: Yonsei University College of Medicine
Jae-Sung Woo: Korea University
Hiroshi Nishimasu: The University of Tokyo
Sung-Rae Cho: Yonsei University College of Medicine
Sungroh Yoon: Seoul National University
Hyongbum Henry Kim: Yonsei University College of Medicine

Nature Communications, 2021, vol. 12, issue 1, 1-8

Abstract: Abstract Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold (range, 0.99- to 2.6-fold) higher efficiencies than PE2; furthermore, at sites where PE2-induced prime editing is very inefficient (efficiency

Date: 2021
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DOI: 10.1038/s41467-021-25928-2

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