Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms

Nicolas Flaugnatti, Sandrine Isaac, Leonardo F. Lemos Rocha, Sandrine Stutzmann, Olaya Rendueles, Candice Stoudmann, Nina Vesel, Marc Garcia-Garcera, Amandine Buffet, Thibault G. Sana, Eduardo P. C. Rocha and Melanie Blokesch ()
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Nicolas Flaugnatti: Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)
Sandrine Isaac: Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)
Leonardo F. Lemos Rocha: Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)
Sandrine Stutzmann: Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)
Olaya Rendueles: Institut Pasteur, CNRS, UMR3525
Candice Stoudmann: Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)
Nina Vesel: Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)
Marc Garcia-Garcera: University of Lausanne
Amandine Buffet: Institut Pasteur, CNRS, UMR3525
Thibault G. Sana: Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)
Eduardo P. C. Rocha: Institut Pasteur, CNRS, UMR3525
Melanie Blokesch: Laboratory of Molecular Microbiology, Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)

Nature Communications, 2021, vol. 12, issue 1, 1-13

Abstract: Abstract While the major virulence factors for Vibrio cholerae, the cause of the devastating diarrheal disease cholera, have been extensively studied, the initial intestinal colonization of the bacterium is not well understood because non-human adult animals are refractory to its colonization. Recent studies suggest the involvement of an interbacterial killing device known as the type VI secretion system (T6SS). Here, we tested the T6SS-dependent interaction of V. cholerae with a selection of human gut commensal isolates. We show that the pathogen efficiently depleted representative genera of the Proteobacteria in vitro, while members of the Enterobacter cloacae complex and several Klebsiella species remained unaffected. We demonstrate that this resistance against T6SS assaults was mediated by the production of superior T6SS machinery or a barrier exerted by group I capsules. Collectively, our data provide new insights into immunity protein-independent T6SS resistance employed by the human microbiota and colonization resistance in general.

Date: 2021
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DOI: 10.1038/s41467-021-26041-0

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