Self-assembled multifunctional neural probes for precise integration of optogenetics and electrophysiology

Liang Zou, Huihui Tian, Shouliang Guan, Jianfei Ding, Lei Gao, Jinfen Wang and Ying Fang ()
Additional contact information
Liang Zou: CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
Huihui Tian: CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
Shouliang Guan: CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
Jianfei Ding: CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
Lei Gao: CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
Jinfen Wang: CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology
Ying Fang: CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology

Nature Communications, 2021, vol. 12, issue 1, 1-9

Abstract: Abstract Optogenetics combined with electrical recording has emerged as a powerful tool for investigating causal relationships between neural circuit activity and function. However, the size of optogenetically manipulated tissue is typically 1-2 orders of magnitude larger than that can be electrically recorded, rendering difficulty for assigning functional roles of recorded neurons. Here we report a viral vector-delivery optrode (VVD-optrode) system for precise integration of optogenetics and electrophysiology in the brain. Our system consists of flexible microelectrode filaments and fiber optics that are simultaneously self-assembled in a nanoliter-scale, viral vector-delivery polymer carrier. The highly localized delivery and neuronal expression of opsin genes at microelectrode-tissue interfaces ensure high spatial congruence between optogenetically manipulated and electrically recorded neuronal populations. We demonstrate that this multifunctional system is capable of optogenetic manipulation and electrical recording of spatially defined neuronal populations for three months, allowing precise and long-term studies of neural circuit functions.

Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (1)

Downloads: (external link)
https://www.nature.com/articles/s41467-021-26168-0 Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26168-0

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-021-26168-0

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().