Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth

Pamela Pruski, Gonçalo D. S. Correia, Holly V. Lewis, Katia Capuccini, Paolo Inglese, Denise Chan, Richard G. Brown, Lindsay Kindinger, Yun S. Lee, Ann Smith, Julian Marchesi, Julie A. K. McDonald, Simon Cameron, Kate Alexander-Hardiman, Anna L. David, Sarah J. Stock, Jane E. Norman, Vasso Terzidou, T. G. Teoh, Lynne Sykes, Phillip R. Bennett, Zoltan Takats () and David A. MacIntyre ()
Additional contact information
Pamela Pruski: Faculty of Medicine Imperial College London
Gonçalo D. S. Correia: Faculty of Medicine Imperial College London
Holly V. Lewis: March of Dimes Prematurity Research Centre at Imperial College London
Katia Capuccini: March of Dimes Prematurity Research Centre at Imperial College London
Paolo Inglese: Faculty of Medicine Imperial College London
Denise Chan: March of Dimes Prematurity Research Centre at Imperial College London
Richard G. Brown: Digestion and Reproduction, Imperial College London
Lindsay Kindinger: Digestion and Reproduction, Imperial College London
Yun S. Lee: March of Dimes Prematurity Research Centre at Imperial College London
Ann Smith: University West of England
Julian Marchesi: Faculty of Medicine Imperial College London
Julie A. K. McDonald: Imperial College London
Simon Cameron: Faculty of Medicine Imperial College London
Kate Alexander-Hardiman: Faculty of Medicine Imperial College London
Anna L. David: University College London
Sarah J. Stock: University of Edinburgh
Jane E. Norman: University of Edinburgh
Vasso Terzidou: March of Dimes Prematurity Research Centre at Imperial College London
T. G. Teoh: Imperial College Healthcare NHS Trust
Lynne Sykes: March of Dimes Prematurity Research Centre at Imperial College London
Phillip R. Bennett: March of Dimes Prematurity Research Centre at Imperial College London
Zoltan Takats: Faculty of Medicine Imperial College London
David A. MacIntyre: March of Dimes Prematurity Research Centre at Imperial College London

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of the cervicovaginal metabolome in two independent pregnancy cohorts (VMET, n = 160; 455 swabs; VMET II, n = 205; 573 swabs). By integrating metataxonomics and immune profiling data from matched samples, we show that specific metabolome signatures can be used to robustly predict simultaneously both the composition of the vaginal microbiome and host inflammatory status. In these patients, vaginal microbiota instability and innate immune activation, as predicted using DESI-MS, associated with preterm birth, including in women receiving cervical cerclage for preterm birth prevention. These findings highlight direct on-swab metabolic profiling by DESI-MS as an innovative approach for preterm birth risk stratification through rapid assessment of vaginal microbiota-host dynamics.

Date: 2021
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DOI: 10.1038/s41467-021-26215-w

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