A microfluidic platform for highly parallel bite by bite profiling of mosquito-borne pathogen transmission

Shailabh Kumar, Felix J. H. Hol, Sujit Pujhari, Clayton Ellington, Haripriya Vaidehi Narayanan, Hongquan Li, Jason L. Rasgon and Manu Prakash ()
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Shailabh Kumar: Stanford University
Felix J. H. Hol: Stanford University
Sujit Pujhari: The Pennsylvania State University
Clayton Ellington: Stanford University
Haripriya Vaidehi Narayanan: Stanford University
Hongquan Li: Stanford University
Jason L. Rasgon: The Pennsylvania State University
Manu Prakash: Stanford University

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract Mosquito bites transmit a number of pathogens via salivary droplets deposited during blood-feeding, resulting in potentially fatal diseases. Little is known about the genomic content of these nanodroplets, including the transmission dynamics of live pathogens. Here we introduce Vectorchip, a low-cost, scalable microfluidic platform enabling high-throughput molecular interrogation of individual mosquito bites. We introduce an ultra-thin PDMS membrane which acts as a biting interface to arrays of micro-wells. Freely-behaving mosquitoes deposit saliva droplets by biting into these micro-wells. By modulating membrane thickness, we observe species-dependent differences in mosquito biting capacity, utilizable for selective sample collection. We demonstrate RT-PCR and focus-forming assays on-chip to detect mosquito DNA, Zika virus RNA, as well as quantify infectious Mayaro virus particles transmitted from single mosquito bites. The Vectorchip presents a promising approach for single-bite-resolution laboratory and field characterization of vector-pathogen communities, and could serve as a powerful early warning sentinel for mosquito-borne diseases.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26300-0

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DOI: 10.1038/s41467-021-26300-0

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