Light-triggered and phosphorylation-dependent 14-3-3 association with NON-PHOTOTROPIC HYPOCOTYL 3 is required for hypocotyl phototropism

Lea Reuter, Tanja Schmidt, Prabha Manishankar, Christian Throm, Jutta Keicher, Andrea Bock, Irina Droste-Borel and Claudia Oecking ()
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Lea Reuter: University of Tübingen
Tanja Schmidt: University of Tübingen
Prabha Manishankar: University of Tübingen
Christian Throm: University of Tübingen
Jutta Keicher: University of Tübingen
Andrea Bock: University of Tübingen
Irina Droste-Borel: University of Tübingen
Claudia Oecking: University of Tübingen

Nature Communications, 2021, vol. 12, issue 1, 1-15

Abstract: Abstract NON-PHOTOTROPIC HYPOCOTYL 3 (NPH3) is a key component of the auxin-dependent plant phototropic growth response. We report that NPH3 directly binds polyacidic phospholipids, required for plasma membrane association in darkness. We further demonstrate that blue light induces an immediate phosphorylation of a C-terminal 14-3-3 binding motif in NPH3. Subsequent association of 14-3-3 proteins is causal for the light-induced release of NPH3 from the membrane and accompanied by NPH3 dephosphorylation. In the cytosol, NPH3 dynamically transitions into membraneless condensate-like structures. The dephosphorylated state of the 14-3-3 binding site and NPH3 membrane recruitment are recoverable in darkness. NPH3 variants that constitutively localize either to the membrane or to condensates are non-functional, revealing a fundamental role of the 14-3-3 mediated dynamic change in NPH3 localization for auxin-dependent phototropism. This regulatory mechanism might be of general nature, given that several members of the NPH3-like family interact with 14-3-3 via a C-terminal motif.

Date: 2021
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DOI: 10.1038/s41467-021-26332-6

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