Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins

Claudia M. Fusco, Kristina Desch, Aline R. Dörrbaum, Mantian Wang, Anja Staab, Ivy C. W. Chan, Eleanor Vail, Veronica Villeri, Julian D. Langer and Erin M. Schuman ()
Additional contact information
Claudia M. Fusco: Max Planck Institute for Brain Research
Kristina Desch: Max Planck Institute for Brain Research
Aline R. Dörrbaum: Max Planck Institute for Brain Research
Mantian Wang: Max Planck Institute for Brain Research
Anja Staab: Max Planck Institute for Brain Research
Ivy C. W. Chan: Max Planck Institute for Brain Research
Eleanor Vail: Max Planck Institute for Brain Research
Veronica Villeri: Max Planck Institute for Brain Research
Julian D. Langer: Max Planck Institute for Brain Research
Erin M. Schuman: Max Planck Institute for Brain Research

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract Owing to their morphological complexity and dense network connections, neurons modify their proteomes locally, using mRNAs and ribosomes present in the neuropil (tissue enriched for dendrites and axons). Although ribosome biogenesis largely takes place in the nucleus and perinuclear region, neuronal ribosomal protein (RP) mRNAs have been frequently detected remotely, in dendrites and axons. Here, using imaging and ribosome profiling, we directly detected the RP mRNAs and their translation in the neuropil. Combining brief metabolic labeling with mass spectrometry, we found that a group of RPs rapidly associated with translating ribosomes in the cytoplasm and that this incorporation was independent of canonical ribosome biogenesis. Moreover, the incorporation probability of some RPs was regulated by location (neurites vs. cell bodies) and changes in the cellular environment (following oxidative stress). Our results suggest new mechanisms for the local activation, repair and/or specialization of the translational machinery within neuronal processes, potentially allowing neuronal synapses a rapid means to regulate local protein synthesis.

Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations: View citations in EconPapers (4)

Downloads: (external link)
https://www.nature.com/articles/s41467-021-26365-x Abstract (text/html)

Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.

Export reference: BibTeX RIS (EndNote, ProCite, RefMan) HTML/Text

Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26365-x

Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/

DOI: 10.1038/s41467-021-26365-x

Access Statistics for this article

Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie

More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().