NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration

Béryl Laplace-Builhé, Audrey Barthelaix, Said Assou, Candice Bohaud, Marine Pratlong, Dany Severac, Gautier Tejedor, Patricia Luz-Crawford, Mai Nguyen-Chi, Marc Mathieu, Christian Jorgensen and Farida Djouad ()
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Béryl Laplace-Builhé: IRMB, Univ Montpellier, INSERM
Audrey Barthelaix: IRMB, Univ Montpellier, INSERM
Said Assou: IRMB, Univ Montpellier, INSERM
Candice Bohaud: IRMB, Univ Montpellier, INSERM
Marine Pratlong: MGX, BCM, Univ Montpellier, CNRS, INSERM
Dany Severac: MGX, BCM, Univ Montpellier, CNRS, INSERM
Gautier Tejedor: IRMB, Univ Montpellier, INSERM
Patricia Luz-Crawford: Universidad de los Andes
Mai Nguyen-Chi: LPHI, Univ Montpellier, CNRS
Marc Mathieu: IRMB, Univ Montpellier, INSERM
Christian Jorgensen: IRMB, Univ Montpellier, INSERM
Farida Djouad: IRMB, Univ Montpellier, INSERM

Nature Communications, 2021, vol. 12, issue 1, 1-14

Abstract: Abstract Fish species, such as zebrafish (Danio rerio), can regenerate their appendages after amputation through the formation of a heterogeneous cellular structure named blastema. Here, by combining live imaging of triple transgenic zebrafish embryos and single-cell RNA sequencing we established a detailed cell atlas of the regenerating caudal fin in zebrafish larvae. We confirmed the presence of macrophage subsets that govern zebrafish fin regeneration, and identified a foxd3-positive cell population within the regenerating fin. Genetic depletion of these foxd3-positive neural crest-derived cells (NCdC) showed that they are involved in blastema formation and caudal fin regeneration. Finally, chemical inhibition and transcriptomic analysis demonstrated that these foxd3-positive cells regulate macrophage recruitment and polarization through the NRG1/ErbB pathway. Here, we show the diversity of the cells required for blastema formation, identify a discrete foxd3-positive NCdC population, and reveal the critical function of the NRG1/ErbB pathway in controlling the dialogue between macrophages and NCdC.

Date: 2021
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DOI: 10.1038/s41467-021-26422-5

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