Structural library and visualization of endogenously oxidized phosphatidylcholines using mass spectrometry-based techniques

Yuta Matsuoka, Masatomo Takahashi, Yuki Sugiura, Yoshihiro Izumi, Kazuhiro Nishiyama, Motohiro Nishida, Makoto Suematsu, Takeshi Bamba and Ken-ichi Yamada ()
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Yuta Matsuoka: Kyushu University
Masatomo Takahashi: Kyushu University
Yuki Sugiura: Keio University School of Medicine
Yoshihiro Izumi: Kyushu University
Kazuhiro Nishiyama: Kyushu University
Motohiro Nishida: Kyushu University
Makoto Suematsu: Keio University School of Medicine
Takeshi Bamba: Kyushu University
Ken-ichi Yamada: Kyushu University

Nature Communications, 2021, vol. 12, issue 1, 1-12

Abstract: Abstract Although oxidized phosphatidylcholines (oxPCs) play critical roles in numerous pathological events, the type and production sites of endogenous oxPCs remain unknown because of the lack of structural information and dedicated analytical methods. Herein, a library of 465 oxPCs is constructed using high-resolution mass spectrometry-based non-targeted analytical methods and employed to detect 70 oxPCs in mice with acetaminophen-induced acute liver failure. We show that doubly oxygenated polyunsaturated fatty acid (PUFA)-PCs (PC PUFA;O2), containing epoxy and hydroxide groups, are generated in the early phase of liver injury. Hybridization with in-vivo 18O labeling and matrix-assisted laser desorption/ionization-tandem MS imaging reveals that PC PUFA;O2 are accumulated in cytochrome P450 2E1-expressing and glutathione-depleted hepatocytes, which are the major sites of liver injury. The developed library and visualization methodology should facilitate the characterization of specific lipid peroxidation events and enhance our understanding of their physiological and pathological significance in lipid peroxidation-related diseases.

Date: 2021
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Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26633-w

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DOI: 10.1038/s41467-021-26633-w

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