Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds

Mitchell Clough, Ichun Anderson Chen, Seong-Wook Park, Allison M. Ahrens, Jeffrey N. Stirman, Spencer L. Smith and Jerry L. Chen ()
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Mitchell Clough: Boston University
Ichun Anderson Chen: Boston University
Seong-Wook Park: Boston University
Allison M. Ahrens: Boston University
Jeffrey N. Stirman: University of North Carolina School of Medicine
Spencer L. Smith: University of California Santa Barbara
Jerry L. Chen: Boston University

Nature Communications, 2021, vol. 12, issue 1, 1-7

Abstract: Abstract Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales.

Date: 2021
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DOI: 10.1038/s41467-021-26737-3

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