Efficient biosynthesis of nucleoside cytokinin angustmycin A containing an unusual sugar system
Le Yu,
Wenting Zhou,
Yixuan She,
Hongmin Ma,
You-Sheng Cai,
Ming Jiang,
Zixin Deng,
Neil P. J. Price () and
Wenqing Chen ()
Additional contact information
Le Yu: Wuhan University
Wenting Zhou: Wuhan University
Yixuan She: Wuhan University
Hongmin Ma: Wuhan University
You-Sheng Cai: Wuhan University
Ming Jiang: Shanghai Jiao Tong University
Zixin Deng: Wuhan University
Neil P. J. Price: National Center for Agricultural Utilization Research
Wenqing Chen: Wuhan University
Nature Communications, 2021, vol. 12, issue 1, 1-11
Abstract:
Abstract Angustmycin A has anti-mycobacterial and cytokinin activities, and contains an intriguing structure in which an unusual sugar with C5′-C6′ dehydration is linked to adenine via an N-glycosidic bond. However, the logic underlying the biosynthesis of this molecule has long remained obscure. Here, we address angustmycin A biosynthesis by the full deciphering of its pathway. We demonstrate that AgmD, C, A, E, and B function as d-allulose 6-phosphate 3-epimerase, d-allulose 6-phosphate pyrophosphokinase, adenine phosphoallulosyltransferase, phosphoribohydrolase, and phosphatase, respectively, and that these collaboratively catalyze the relay reactions to biosynthesize angustmycin C. Additionally, we provide evidence that AgmF is a noncanonical dehydratase for the final step to angustmycin A via a self-sufficient strategy for cofactor recycling. Finally, we have reconstituted the entire six-enzyme pathway in vitro and in E. coli leading to angustmycin A production. These results expand the enzymatic repertoire regarding natural product biosynthesis, and also open the way for rational and rapid discovery of other angustmycin related antibiotics.
Date: 2021
References: View references in EconPapers View complete reference list from CitEc
Citations:
Downloads: (external link)
https://www.nature.com/articles/s41467-021-26928-y Abstract (text/html)
Related works:
This item may be available elsewhere in EconPapers: Search for items with the same title.
Export reference: BibTeX
RIS (EndNote, ProCite, RefMan)
HTML/Text
Persistent link: https://EconPapers.repec.org/RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26928-y
Ordering information: This journal article can be ordered from
https://www.nature.com/ncomms/
DOI: 10.1038/s41467-021-26928-y
Access Statistics for this article
Nature Communications is currently edited by Nathalie Le Bot, Enda Bergin and Fiona Gillespie
More articles in Nature Communications from Nature
Bibliographic data for series maintained by Sonal Shukla () and Springer Nature Abstracting and Indexing ().