A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes

Marion Darnaud (), Filipe Vadder, Pascaline Bogeat, Lilia Boucinha, Anne-Laure Bulteau, Andrei Bunescu, Céline Couturier, Ana Delgado, Hélène Dugua, Céline Elie, Alban Mathieu, Tereza Novotná, Djomangan Adama Ouattara, Séverine Planel, Adrien Saliou, Dagmar Šrůtková, Jennifer Yansouni, Bärbel Stecher, Martin Schwarzer, François Leulier and Andrea Tamellini
Additional contact information
Marion Darnaud: Institut de Recherche Technologique
Filipe Vadder: Université Claude Bernard Lyon 1
Pascaline Bogeat: Institut de Recherche Technologique
Lilia Boucinha: Institut de Recherche Technologique
Anne-Laure Bulteau: Université Claude Bernard Lyon 1
Andrei Bunescu: Institut de Recherche Technologique
Céline Couturier: Institut de Recherche Technologique
Ana Delgado: Institut de Recherche Technologique
Hélène Dugua: Institut de Recherche Technologique
Céline Elie: Institut de Recherche Technologique
Alban Mathieu: Institut de Recherche Technologique
Tereza Novotná: Institute of Microbiology of the Czech Academy of Sciences
Djomangan Adama Ouattara: Institut de Recherche Technologique
Séverine Planel: Institut de Recherche Technologique
Adrien Saliou: Institut de Recherche Technologique
Dagmar Šrůtková: Institute of Microbiology of the Czech Academy of Sciences
Jennifer Yansouni: Institut de Recherche Technologique
Bärbel Stecher: Ludwig-Maximilians-University of Munich
Martin Schwarzer: Institute of Microbiology of the Czech Academy of Sciences
François Leulier: Institut de Recherche Technologique
Andrea Tamellini: Institut de Recherche Technologique

Nature Communications, 2021, vol. 12, issue 1, 1-21

Abstract: Abstract Mus musculus is the classic mammalian model for biomedical research. Despite global efforts to standardize breeding and experimental procedures, the undefined composition and interindividual diversity of the microbiota of laboratory mice remains a limitation. In an attempt to standardize the gut microbiome in preclinical mouse studies, here we report the development of a simplified mouse microbiota composed of 15 strains from 7 of the 20 most prevalent bacterial families representative of the fecal microbiota of C57BL/6J Specific (and Opportunistic) Pathogen-Free (SPF/SOPF) animals and the derivation of a standardized gnotobiotic mouse model called GM15. GM15 recapitulates extensively the functionalities found in the C57BL/6J SOPF microbiota metagenome, and GM15 animals are phenotypically similar to SOPF or SPF animals in two different facilities. They are also less sensitive to the deleterious effects of post-weaning malnutrition. In this work, we show that the GM15 model provides increased reproducibility and robustness of preclinical studies by limiting the confounding effect of fluctuation in microbiota composition, and offers opportunities for research focused on how the microbiota shapes host physiology in health and disease.

Date: 2021
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DOI: 10.1038/s41467-021-26963-9

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